Fast PCR amplification of AmpFlSTR Identifiler |
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| Authors: | Kazuhiko Tsukada Maiko Nojiri Yoshinobu Kurasawa Koichi Kasahara |
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| Affiliation: | Department of Forensic Medicine, Criminal Investigation Laboratory, Nagano Pref. Police H.Q., 3916 Nishijo, Matsushiro-Machi, Nagano-shi, Nagano, 381-1232, Japan |
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| Abstract: | ![]()
To increase throughput for DNA typing, we examined fast PCR cycling using AmpFlSTR Identifiler by three methods. In this study, we reduced PCR running times by 1/3 to 2/3 (approximately 1-2 h). This means DNA typing, including PCR reactions, can be completed within a timeframe ranging from 90 min to 2 h and 30 min. |
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| Keywords: | Fast PCR AmpFlSTR Identifiler QIAGEN fast cycling PCR 9800 fast thermal cycler |
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