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AmpFlSTR MiniFiler™ PCR amplification kit: The new miniSTR multiplex kit |
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| Authors: | L Andrade AM Bento M Carvalho C Oliveira V Lopes F Corte-Real |
| Institution: | a Forensic Genetic Service, Central Region Department, National Institute of Legal Medicine, Portugal b Department of Legal Medicine, Faculty of Medicine, University of Cádiz, Spain c National Institute of Legal Medicine, Portugal |
| Abstract: | The AmpFlSTR® MiniFiler™ PCR amplification kit (Applied Biosystems), a new available 8-miniSTR and the sex determining marker Amelogenin multiplex, includes the most common problematic loci (above 200 bp) of the AmpFlSTR® Identifiler™ PCR amplification kit: FGA, D21S11, D18S51, D13S317, D7S820, D16S539, CSF1PO and D2S1338.Several casework samples with different DNA contents were tested.Results allowed to complete partial Identifiler™ profiles and additional information was achieved in low copy number (LCN) samples, revealing that this miniSTR kit can improve identification of compromised samples. |
| Keywords: | MiniSTRs LCN |
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