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New autosomal STR loci
Authors:J.M. Butler  C.R. Hill  A.E. Decker  M.C. Kline  P.M. Vallone
Affiliation:National Institute of Standards and Technology, Biochemical Science Division, Gaithersburg, MD 20899-8311, USA
Abstract:
Additional STR loci can be beneficial for a number of human identity, forensic casework, and DNA database applications. The marker selection and characterization process applied at NIST in developing these new loci and assays are described along with concordance testing results from non-overlapping PCR primers. A 23plex for simultaneous amplification of 22 autosomal STR loci and an amelogenin sex-typing assay is also demonstrated.
Keywords:STR   Short tandem repeat   NIST   D10S1248   D2S441   D22S1045   Multiplex assays   MiniSTRs   Degraded DNA   Forensic DNA typing
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