| DNA纹印技术的初步研究——用PYNH24探针分析MspI的RFLP |
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| 引用本文： | 李伯龄,倪锦堂,储晓兰,叶健.DNA纹印技术的初步研究——用PYNH24探针分析MspI的RFLP[J].中国法医学杂志,1988(1). |
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| 作者姓名： | 李伯龄 倪锦堂 储晓兰 叶健 |
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| 作者单位： | 公安部第二研究所，公安部第二研究所，公安部第二研究所，公安部第二研究所 |
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| 摘 要： | 应用 Southern 印迹杂交技术,分析了人体基因组 DNA MspI 酶解物与特异探针 PYNH24杂交的限制酶切片段长度多态性,获得清晰易读的杂交图谱。50个不相关个体血样中未发现相同图谱,显示了个体特异性,为法医生物物证检验工作提供了新的分析手段。
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| 关 键 词： | 多态性 探针 DNA 纹印 法医生物学 |
PRIMARY RESARCH ON DNA PRINT IN CHINESE——ANALYSIS OF MspI RFLPS USING PYNH 24 AS A PROBE |
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| Li Boling Ni Jintang Chu Xiaolan Ye Jian Instute of Forensic Science ministry of Public Security,Beijing.PRIMARY RESARCH ON DNA PRINT IN CHINESE——ANALYSIS OF MspI RFLPS USING PYNH 24 AS A PROBE[J].Chinese Journal of Forensic Medicine,1988(1). |
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| Authors: | Li Boling Ni Jintang Chu Xiaolan Ye Jian Instute of Forensic Science ministry of Public Security Beijing |
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| Abstract: | Restriction fragment length Polymorphism of genome DNA in Chinese
was analyzed using southern blot technique and hybridization of MspI
enzymic hydrolytic production with specific probe PYNH24.A clear hybrid-
ization pattern was obtained.No same pattern was seen in 50 unrelated ind-
ividuals.It showed a high individual specifity.It provides a new method
for the examination of forensic biological materials. |
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| Keywords: | Polymorphism Probe DNA finoerprint Forensic biology |
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