| 血细胞X染色体失活偏移与女性体质关联性研究 |
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| 作者姓名： | 李刚 梁红娟 巩丽 朱燕波 苏勤 陈忠平 |
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| 作者单位： | 李刚,陈忠平,LI Gang,CHEN Zhong-ping(华南肿瘤学国家重点实验室中山大学肿瘤防治中心,广东,广州510060);梁红娟,巩丽,苏勤,LIANG Hong-juang,GONG Li,SU Qin(中国人民解放军第四军医大学唐都医院);朱燕波,ZHU yan-bo(北京中医药大学管理学院)
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| 摘 要： | 目的:探索血细胞X染色体失活偏移(skewed X-chromosomal inactivation, SXCI)与女性体质的关联性.方法:对15岁以上、获知情同意的1 103例健康女性,采用标准化中医体质量表计算各亚量表分数,判定个体为平和质或偏颇体质.对上述个体的外周血标本,提取基因组DNA,经Hpa Ⅱ消化后,PCR扩增雄激素受体(androgen receptor, AR)基因,产物经变性聚丙烯酰胺凝胶电泳,银染显示结果,比较Hpa Ⅱ消化前后AR基因扩增产物的强度差异,计算其校正比值(CR).结果:以CR≥3为判断SXCI标准时,平和质、气虚质、阳虚质、阴虚质、痰湿质、湿热质、瘀血质、气郁质、特禀质组间SXCI的发生率分别为8.9%(33/370)、32.0%(48/150)、8.1%(5/62)、11.3%(6/53)、18.4%(16/87)、22.9%(19/83)、10.9%(5/46)、29.0%(29/100)和8.0%(4/50),发生率间有显著性差异(χ2=61.071,P<0.05).气虚质、痰湿质、湿热质与气郁质SXCI发生率较高,分别为32.0%(48/150)、18.4%(16/87)、22.9%(19/83)和29.0%(29/100).分型与SXCI关联系数为0.244(P<0.05),差异有统计学意义.结论:血细胞SXCI与女性体质关联.
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| 关 键 词： | X染色体失活偏移 中医体质 量表 |
Skewed X-chromosomal Inactivation in Blood Cells Is Associated with Body Constitution of Traditional Chinese Medicine in Females |
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| Authors: | LI Gang LIANG Hong-juang GONG Li ZHU yan-bo SU Qin CHEN Zhong-ping |
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| Institution: | LI Gang,LIANG Hong-juang,GONG Li,ZHU yan-bo,SU Qin,CHEN Zhong-ping(State Key Laboratory for Cancer Research in Southern China & Cancer Center,Sun Yat-sen University,Guangdong Guangzhou 510060,China) |
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| Abstract: | Objective:To elucidate the correlation between skewed X chromosome inactivation(SXCI)and body constitution of traditional Chinese medicine(TCM) in females.Methods:The types of TCM body constitution of 1 103 normal females above 15 years old,from which a written informed consent was obtained,were assessed using the standard Constitution in Chinese Medicine Questionnaire(CCMQ) based on the scores of sub-CCMQ.Meanwhile,DNA was isolated from mononuclear blood cells from the subjects.Exon 1 of androgen receptor(... |
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| Keywords: | Skewed X-chromosomal inactivation Constitution in Chinese Medicine Questionnaire Body constitution in Chinese Traditional Medicine |
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