可卡因染毒致小鼠免疫功能损伤的影响

目的考察可卡因染毒对小鼠免疫功能的影响。方法615纯系雄性小鼠20只，随机均分为实验组和对照组；实验组小鼠以30mg／kg盐酸可卡因每天1次进行腹腔连续注射，空白组给予相同量盐水同法注射盐水；第30d处死小鼠，测量体重与脏器系数，用四甲基偶氮唑蓝（MTr）法检测脾细胞、胸腺细胞与腹腔巨噬细胞（PMφs）的增殖活性；用生物活性检测法检测IL-1与IL-2的活性；用ELISA法检测IFN-γ／与TNF—α含量。结果实验组小鼠脾细胞、胸腺细胞与PMφs的增殖反应明显降低；脾细胞培养上清液中IL-2与IFN-γ活性明显降低；PMφs培养上清中IL-1与TNF—α活性明显降低；体重、脾脏与胸腺系数也明显降低；上述指标较之对照组均有显著性差异（P〈0．01）。结论在活体条件下可卡因染毒对小鼠免疫系统功能有明显抑制作用。

The effect of cocaine exposure on immune function of mouse in vivo

Objective To investigate the effect of Cocaine exposure on immune function of mouse. Methods The 615 male mice were randomly divided into experimental group and control group. Cocaine hydrochloride was administered intraperitoneally at a dose of 30 mg/kg once a day in experimental mice, and blank group were given the same dose of normal saline as controls. After 30 days, the mice were killed for the measurement of body weight and viscera codfficient. Meanwhile, the MTT method was used to test the proliferation activity of the splenocytes, thymocytes and PMφs cells. The activity of IL-1 and IL-2, the content of IFN-γ and TNF-α were measured by biological assays and ELISA, respectively. Results In experimental mice, the proliferation reactions of splenocytes, thymoeytes and PMφs were obviously decreased. The activity of IL-2 and IFN-γcontent in the splenocyte culture supernatant were significantly reduced. The activity of IL- l and TNF-α content in the PMφs culture supernatant showed the same changes. The weight and the coefficient of spleen and thymus in experimental mice were obviously decreased （ P 〈 0. 01 ）. Conclusion Cocaine hydrochloride can significantly inhibit the immune function of mouse in vivo.