| 快速溶剂萃取-GC／MS法测定全血中沙蚕毒素 |
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| 引用本文： | 唐 磊,姚焕焕,罗永此,李晓飞,徐 琛.快速溶剂萃取-GC／MS法测定全血中沙蚕毒素[J].中国法医学杂志,2014(1):53-55. |
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| 作者姓名： | 唐 磊 姚焕焕 罗永此 李晓飞 徐 琛 |
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| 作者单位： | 杭州市公安局刑事科学技术研究所,浙江杭州310004 |
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| 摘 要： | 目的采用快速溶剂萃取-GC／MS分析方法检验血液中杀虫双代谢产物沙蚕毒素。方法样本用硅藻土、中性三氧化二铝处理，用乙酸乙酯：环己烷：丙酮（2：2：1）进行萃取，GC／MS方法进行测定，并考察实验最佳条件。结果沙蚕毒素在50～2000ng／mL范围内线性关系良好，相关系数为0．999，检测限为15ng／mL，平均回收率为98．22％，相对标准偏差为4．6％。结论速溶剂萃取-GC／MS分析方法简便，快速，准确、灵敏度高，可在血中沙蚕毒素检验中选用。
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| 关 键 词： | 法医毒物分析 沙蚕毒素 快速溶剂萃取 气相色谱一质谱 全血 |
GC/MS determination of nereistoxin in whole blood with accelerated solvent extraction |
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| Tang Lei,Yao Huanhuan,Luo Yongci,Li Xiaofei,Xu Chen.GC/MS determination of nereistoxin in whole blood with accelerated solvent extraction[J].Chinese Journal of Forensic Medicine,2014(1):53-55. |
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| Authors: | Tang Lei Yao Huanhuan Luo Yongci Li Xiaofei Xu Chen |
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| Institution: | (Hangzhou Institute of Criminal Science ＆ Technology, Hangzhou 310004, China) |
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| Abstract: | Objective To establish a method for determination of nereistoxin in human plasma by accelerated solvent extraction gas-chromatography. Methods We extracted nereistoxin from plasma by ethyl acetate ： cyclohenane ： acetone （2 ： 2 ： 1 ） and detected it with GC/MS. Results The linear range of nereistoxin was 50 - 2000ng/mL（R2 = 0. 999）, the minimal detection limits （LOD） was 15 ng/mL, and the average recoveries of the nereistoxin was 98.22%, respectively. Conclusion The method has high sensitivity and good reproducibility without the endogenous interference. The result is accurate and reliable with a wide linear range. It can be used in forensic toxicological analysis. |
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| Keywords: | forensic toxicological analysis nereistoxin accelerated solvent extraction GC/MS wholeblood |
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