| 家兔死后心血氧化还原电位值变化与死亡时间的关系 |
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| 引用本文： | 杨天潼,于永光,白敬,张海东,孙婷怡,Mary G. Ripple,David R. Fowler,李玲.家兔死后心血氧化还原电位值变化与死亡时间的关系[J].法医学杂志,2013(5):321-324,329. |
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| 作者姓名： | 杨天潼 于永光 白敬 张海东 孙婷怡 Mary G. Ripple David R. Fowler 李玲 |
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| 作者单位： | [1]中国政法大学教育部证据科学重点实验室,北京100040 [2]北京交通大学理学院,北京100044 [3]华中科技大学同济医学院法医学系,湖北武汉430030 [4]马里兰州法医局,美国马里兰州巴尔的摩21223 |
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| 基金项目： | 国家自然科学基金青年科学基金资助项目（81001349）;教育部长江学者和创新团队发展计划项目（IRT0956） |
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| 摘 要： | 目的研究在不同温度条件下家兔死后心血氧化还原电位(oxidation reduction potential,ORP)值变化与死亡时间(postmortem interval,PMI)的关系。方法随机将48只家兔分为6组,以空气栓塞法处死后,取家兔右心室血液分别置于10、15、20、25、30和35℃温度下的水浴中,自取血即刻(0h)至死后132h内,每4 h用PB-21型电化学分析仪测定样本的ORP值。应用SPSS 17.0软件建立曲线回归方程,再应用MATLAB7.10.0软件建立曲面方程和三维曲面图。结果不同温度条件下家兔心血ORP值变化与PMI具有高度相关性,温度高时,ORP值上升明显,温度低时,ORP值上升缓慢,并获得拟合的曲面方程以及三维曲面图。结论拟合的ORP值与PMI的曲面方程及三维曲面图可进行温度变化条件下的PMI推断。
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| 关 键 词： | 法医病理学 死亡时间 氧化还原电位 差值函数 兔 |
Correlation between the Changes of Oxidation Reduction Potential Values and Post-mortem Interval of Heart Blood in Rabbits after Death |
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| YANG Tian-tong,YU Yong-guang,BAI Jing,ZHANG Hai-dong,SUN Ting-yi,Mary G. Ripple,David R. Fowler,LI Ling.Correlation between the Changes of Oxidation Reduction Potential Values and Post-mortem Interval of Heart Blood in Rabbits after Death[J].Journal of Forensic Medicine,2013(5):321-324,329. |
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| Authors: | YANG Tian-tong YU Yong-guang BAI Jing ZHANG Hai-dong SUN Ting-yi Mary G Ripple David R Fowler LI Ling |
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| Institution: | 1. China University of Political Science and Law, Key Laboratory of Evidence Science, Ministry of Education, Beijing 100040, Chino,＂ 2. School of Science, Bei]ing Jiaotong University, Beijing 100044, Chino,＂ 3. Faculty of Forensic Medicine, Tongi Medical College, Huazhong University of Science and Technology, Wuhan 430030, Chino; 4. Office of the Chief Medical Examiner of Maryland, Baltimore 21223, USA ) |
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| Abstract: | Objective To investigate correlation between the changes of oxidation reduction potential （ORP ） values of heart blood in rabbits after death and postm ortem interval （PMI） at different temperatures. Meth-ods Forty-eight rabbits were random ly divided into 6 groups and sacrificed by air em bolism . Blood sam-ples were taken from the right ventricle of each rabbit and stored at different temperatures of 10, 15, 20, 25, 30and 35℃, respectively. Every 4 hours from 0h to 132 hpostm ortem , the ORP values of the blood samples were measured at different intervals by PB-21 electrochem ical analyzer. The curvilinear regres-sion equation was established by SPSS 17.0software. The surface equation and 3D surface diagram were established by MATL A B7.10.0software. Results The ORP values at different temperatures of heart blood in rabbits were highly correlated with the PMI. The ORP values rised obviously when the temper-ature was high and rised slowly when the temperature was low. The surface equation and 3D surface diagram were obtained. Conclusion The surface equation and 3D surface diagram of ORP values and PMI may be used for PMI estimation at different temperatures. |
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| Keywords: | forensic pathology postm ortem interval oxidation reduction potential interpolation function rabbits |
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