| 吗啡戒断大鼠中脑腹侧背盖区GAP-43的表达变化 |
| |
| 引用本文： | 罗良鸣,吴玉锋,倪维成,朱华.吗啡戒断大鼠中脑腹侧背盖区GAP-43的表达变化[J].法医学杂志,2013(5):330-332,336. |
| |
| 作者姓名： | 罗良鸣 吴玉锋 倪维成 朱华 |
| |
| 作者单位： | [1]温州医学院法医学系,浙江温州325035 [2]温州市公安局刑事科学技术研究所,浙江温州325000 [3]诸暨市公安局刑侦大队,浙江诸暨311800 [4]平阳县公安局刑事技术室,浙江平阳325400 |
| |
| 基金项目： | 浙江省温州市科技局基金资助项目（Y20090237）；浙江省公安厅重大科研资助项目 |
| |
| 摘 要： | 目的观察吗啡戒断大鼠中脑腹侧背盖区生长相关蛋白-43(growth associated protein-43,GAP-43)在不同时程中的表达,探讨GAP-43在吗啡戒断记忆中的作用。方法采用腹腔递增注射盐酸吗啡并自然戒断的方法建立吗啡依赖1周、2周和4周戒断模型,运用免疫组织化学染色观察中脑腹侧背盖区GAP-43的蛋白表达,并用Image-Pro Plus 5.1图像分析软件对结果进行分析。结果中脑腹侧背盖区GAP-43的蛋白表达随依赖时间的延长表现为先降低再升高(P0.01)。结论 GAP-43可能在中脑腹侧背盖区参与了吗啡戒断记忆。
|
| 关 键 词： | 法医病理学 吗啡依赖 生长相关蛋白- 大鼠 |
Expression of GAP-43 in Midbrain Ventral Tegmental Area of Morphine With-drawal Rats |
| |
| LUO Liang-ming,WU Yu-feng,NI Wei-cheng,ZHU Hua.Expression of GAP-43 in Midbrain Ventral Tegmental Area of Morphine With-drawal Rats[J].Journal of Forensic Medicine,2013(5):330-332,336. |
| |
| Authors: | LUO Liang-ming WU Yu-feng NI Wei-cheng ZHU Hua |
| |
| Institution: | 1. Department of Forensic Medicine, Wenzhou Medical College, Wenzhou 325035, China; 2. Institute of Criminal Science and Technology, Wenzhou Public Security Bureau, Wenzhou 325000, China; 3. Criminal Investigation Team, Zhuji Public Security Bureau, Zhuji 311800, China,＂ 4. Department of Criminal Tech- nology, Pingyang Public Security Bureau, Pingyang 325400, China) |
| |
| Abstract: | Objective To observe the protein expression of grow thassociated protein-43 （GAP-43） in mid-brain ventral tegmental area in morphine withdrawal rats at different time, and to evaluate the effect of GAP-43 on morphine withdrawal memory. Methods Rat models of morphine dependent 1 week, 2 weeks and 4 weeks were established by morphine hydrochloride intraperitoneal injection with increasing doses to establish natural withdrawal. The protein expression of GAP-43 in midbrain ventral tegmental area was observed by im munohistochemical staining and the results were analyzed by Im age-Pro Plus 5.1 im-age analysis system . Results With prolongation of dependent time, the expression of GAP-43 was de-creased then increased in midbrain ventral tegmental area . Conclusion GAP-43 could play arole in morphine withdrawal memory in midbrain ventral tegmental area. |
| |
| Keywords: | forensic pathology morphine dependence grow thassociated protein-43 rats |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
