电针对糖尿病大鼠胰腺组织CHOP、Bax、Bcl-2蛋白表达及胰腺超微结构的影响

目的观察针刺对糖尿病大鼠胰腺组织内质网应激及细胞凋亡的影响。方法高脂高糖饲料喂养加低剂量链脲佐菌素腹腔注射复制糖尿病大鼠模型,按血糖分层将模型复制成功的大鼠随机分为模型组、针刺组和二甲双胍组,另设空白组。干预4周后检测随机血糖、大鼠胰腺组织CHOP、Bcl-2、Bax蛋白含量,观察胰腺细胞超微结构。结果模型复制后,模型复制组随机血糖显著高于空白组(P0.05)。针刺组和二甲双胍组随机血糖均较治疗前明显降低(P0.05);与模型组比较,针刺组随机血糖水平呈降低趋势(P0.05)。模型组CHOP、Bax蛋白含量明显高于空白组(P0.05),Bcl-2蛋白含量显著低于空白组(P0.05);模型组、针刺组和二甲双胍组CHOP、Bax蛋白含量比较,差异无统计学意义(P0.05);针刺组和二甲双胍组Bcl-2蛋白含量均显著高于模型组(P0.05)。针刺组和二甲双胍组胰腺细胞线粒体排列整齐,在空泡变性,线粒体嵴断裂,粗面内质网变形,糖原颗粒减少方面,其异常变化较模型组明显减轻。结论针刺可通过改善糖尿病大鼠胰腺组织内质网应激,良性调节Bcl-2、Bax蛋白含量,从而保护胰腺细胞。

Effects of Electroacupuncture on Protein Expression of CHOP, Bax, and Bcl-2 in Pancreatic Tissue and Ultrastructure of Pancreas in Diabetic Rats

Objective To observe the effects of acupuncture on endoplasmic reticulum stress and cell apoptosis in the pancreatic tissue of diabetic rats. Methods A rat model of diabetes was induced by high-fat and high-sugar feeding and intraperitoneal injection of low-dose streptozotocin. The model rats were randomly divided into model group, acupuncture group, and metformin group according to blood glucose stratification; in addition, healthy rats were assigned to blank group. After 4 weeks of treatment, the levels of random blood glucose and proteins (Bax, Bcl-2, and CHOP) were determined, and the ultrastructure of pancreas was observed. Results After the model was established, the content of random blood glucose in the model group was significantly higher than that in the blank group (P<0.05). The acupuncture group and metformin group showed significant decreases in random blood glucose after treatment (P<0.05); compared with the model group, the acupuncture group showed a nonsignificant decrease in random blood glucose (P>0.05). Compared with the blank group, the model group had significantly higher levels of CHOP and Bax (P<0.05) but a significantly lower Bcl-2 level (P<0.05); there were no significant differences in the content of CHOP and Bax between the model group, acupuncture group, and metformin group (P>0.05); the content of Bcl-2 in the acupuncture group and metformin group was significantly lower than that in the model group (P<0.05). When compared with the model group, the acupuncture group and metformin group had significantly reduced abnormal changes with respect to vacuolar degeneration, rupture of mitochondrial cristae, rough endoplasmic reticulum deformation, and reduction in glycogen granules. Conclusion Acupuncture can improve the endoplasmic reticulum stress in the pancreas of diabetic rats and regulate the content of Bcl-2 and Bax, thus protecting pancreatic cells.