| 多指标正交试验优选炮苍耳子中酚酸类成分的提取工艺 |
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| 作者姓名： | 李钰馨 韩燕全 洪燕 邓龙飞 汪永忠 高家荣 夏伦祝 |
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| 作者单位： | 安徽中医药大学,安徽合肥,230012;安徽中医药大学第一附属医院 国家中医药管理局中药制剂三级实验室,安徽合肥,230031 |
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| 基金项目： | 国家中医药重点学科临床中药学建设项目（国中医药人教发［2012］32号） |
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| 摘 要： | 目的 优选苍耳子中酚酸类成分的最佳提取工艺。方法 以苍耳子浸膏得率、5种酚酸类成分及总酚酸的含量为指标，通过正交试验设计，优选出苍耳子中酚酸类成分的最佳水提取工艺。结果 所优选出的最佳提取工艺为10倍量水，浸泡30 min，提取3次，每次90 min。其中提取次数对提取工艺的影响，差异具有统计学意义（P＜0.05）。结论 所选用的提取工艺稳定、简便、可靠，为进一步提取苍耳子中酚酸类成分提供实验参考。
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| 关 键 词： | 苍耳子 酚酸 提取工艺 正交试验 |
Optimization of Extraction of Phenolic Acids from Fructus Xanthii by Multi- index Orthogonal Experiment |
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| Institution: | 1.Anhui University of Chinese Medicine, Anhui Hefei 230012, China; 2. The First Affiliated Hospital of Anhui University of Chinese Medicine & Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of Traditional Chinese Medicine, Anhui Hefei 230031, China |
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| Abstract: | Objective To investigate the optimal extraction technique for phenolic acids from Fructus Xanthii. Methods With the yield of Fructus Xanthii extract and content of five phenolic acids and total phenolic acids as indices, an orthogonal experiment was designed to figure out the optimal aqueous extraction technique for phenolic acids from Fructus Xanthii. Results The optimal extraction was performed by 30- min soaking using 10 volumes of water and 90- min extraction three times. The number of extraction cycles had a significant impact on the extraction technique (P<0.05). Conclusion The optimized extraction technique is stable, simple, and reliable to provide an experimental basis for further extraction of phenolic acids from Fructus Xanthii. |
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| Keywords: | Fructus Xanthii phenolic acids extraction technique orthogonal experiment |
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