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牡丹皮药材规格等级初探
作者姓名:张雨凤  王军  方成武  李静  吴计划
作者单位:安徽中医药大学药学院,安徽合肥,230031;亳州职业技术学院药学院,安徽亳州 236800;安徽省中医药科学院亳州中医药研究所,安徽亳州 236800;安徽中医药大学药学院,安徽合肥230031;亳州职业技术学院药学院,安徽亳州 236800;安徽省中医药科学院亳州中医药研究所,安徽亳州 236800;安徽省中医药科学院亳州中医药研究所,安徽亳州 236800;亳州食品药品检验所,安徽亳州 236800;亳州食品药品检验所,安徽亳州 236800;北京同仁堂安徽中药材有限公司,安徽铜陵244000
基金项目:国家“十二五”科技支撑计划(2011BAI04B05);安徽省教育厅自然科学研究重点项目(KJ2015A388);亳州市创新创业领军人才科研团队项目(亳组(2014)21)
摘    要:目的 探索牡丹皮中主要活性成分的积累量与根的粗细、根的组织结构之间的相关性,为评价牡丹皮质量及牡丹皮药材规格等级的划分提供科学依据。方法 采集安徽省亳州栽培5年生凤丹根系,将根系中的根依据粗细、长度及部位分别进行划分。同时收集市场常见不同规格的牡丹皮商品。采用高效液相色谱法同时测定各样品中芍药苷和丹皮酚含量。结果 在不同等级中,四等牡丹皮中芍药苷含量最高,一等牡丹皮中丹皮酚含量最高;芍药苷含量在主根中最少,在支根中最多;市场上连丹比刮丹中芍药苷含量高,刮丹比连丹中丹皮酚含量高。结论 细的牡丹皮中芍药苷含量反而高,由此推测芍药苷在周皮中积累量较多。由于芍药苷和丹皮酚积累量的趋势相反,因此牡丹皮质量标准有待进一步研究。

关 键 词:牡丹皮  凤丹根系  规格等级  芍药苷  丹皮酚

A Preliminary Study of Specification and Grade of Cortex Moutan
Institution:1.School of Pharmacy, Anhui University of Chinese Medicine, Anhui Hefei 230031, China;2. School of Pharmacy, Bozhou Vocational and Technical College, Anhui Bozhou 236800, China;3. Bozhou Institute of Chinese Medicine, Anhui Academy of Chinese Medicine, Anhui Bozhou 236800, China; 4. Bozhou Institute for Food and Drug Control, Anhui Bozhou 236800, China;5. Beijing Tongrentang Traditional Chinese Medicinal Materials Co., Ltd. in Anhui, Anhui Tongling 244000, China
Abstract:Objective To explore the correlation of the accumulation of the main active ingredients in Cortex Moutan with the thickness and tissue structure of root, and to provide a scientific basis for quality evaluation of Cortex Moutan and its specification and grade classification. Methods The root system of five-year-old Paeonia ostii was collected, and the roots in the root system were classified according to thickness, length, and position. Commercially available Cortex Moutan with different specifications was collected from the market. The content of paeoniflorin and paeonal in each sample was determined using high-performance liquid chromatography. Results Among Cortex Moutan of different grades, grade 4 Cortex Moutan had the highest content of paeoniflorin, while grade 1 Cortex Moutan had the highest content of paeonal. The content of paeoniflorin was lowest in the main root and highest in lateral roots. In the market, the unscraped Cortex Moutan had higher content of paeoniflorin but lower content of paeonal than the scraped Cortex Moutan. Conclusion The thin Cortex Moutan has a relatively high content of paeoniflorin, suggesting that more paeoniflorin accumulates in the periderm. Due to the opposite trends in accumulation of paeoniflorin and paeonal, the quality standard of Cortex Moutan needs further studies.
Keywords:Cortex Moutan  root system of Paeonia ostii  specification and grade  paeoniflorin  paeonal
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