人脑挫伤后bFGF和COX2免疫组化染色观察

目的　观察人脑损伤后bFGF和COX2的动态变化规律 ,建立推断脑挫伤经过时间新方法。方法　应用免疫组织化学染色和图像分析技术 ,对 3 5例重度颅脑损伤案例的脑挫伤灶周边区bFGF和COX2的表达进行观察检测。结果　bFGF阳性细胞在即刻死亡组表达强度最大 ,伤后 12h急剧下降 (P <0 0 1) ,以后逐渐缓慢下降 ;COX2阳性细胞表达于伤后 12h急剧升高 (P <0 0 1) ,伤后 1d达峰值 ,以后逐渐下降 ,在伤后 11d消失。结论　bFGF和COX2的免疫组织化学染色可作为推断人脑挫伤经过时间的参考指标。

An immunohistochemical study of bFGF and COX2 after human brain contusion

Objective Study the changes of bFGF, COX2 after human severe brain injury and find an effective method for estimating time interval after brain injury.Method 35 brain tissue samples of severe brain injury were examined using immunohistochemical staining and image analysis technique to evaluate the expression of bFGF and COX2.Results Maximal bFGF expression was found at 0h after brain contusion. The intensity of bFGF staining decreased remarkably 12h after brain contusion and descended gradually to the minimum on the 11th day. Expression of COX2 positive cells increased significantly 12h after brain contusion and reached the maximum level one day after brain contusion. Then the expression decreased gradually from the 2nd day to almost aero on the 11th day.Conclusion The changes of bFGF and COX2 were regular along with various survival time after brain contusion so that bFGF and COX2 immunohistochemical staining can be used as a referential data for estimating time interval after human brain contusion.