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| 布鲁氏菌pcDNA3.1-omp17.3基因疫苗的研制 | |
| 引用本文： | 邓小红,曾政,王希良.布鲁氏菌pcDNA3.1-omp17.3基因疫苗的研制[J].中国兽医科学,2007,37(6):505-509. |
| 作者姓名： | 邓小红 曾政 王希良 |
| 作者单位： | 1. 重庆工商大学,药物化学与化学生物学研究中心,重庆,400067 2. 重庆市动物疫病预防控制中心,重庆,401147 3. 军事医学科学院,微生物流行病研究所,北京,100071 |
| 摘 要： | 采用PCR方法扩增了布鲁氏菌17.3 ku外膜蛋白编码基因,并将该基因克隆至真核表达载体pcDNA3.1(+)中,成功构建了真核表达质粒pcDNA3.1-omp17.3。pcDNA3.1-omp17.3转染COS-7细胞后,通过Western-blotting检测到了17.3 ku蛋白的瞬时表达。将pcDNA3.1-omp17.3免疫小鼠,三免后经ELISA、流式细胞仪以及ELISPOT技术检测到pcDNA3.1-omp17.3在小鼠体内诱导产生了以Th1型为主的细胞免疫应答。结果表明,构建的基因疫苗可作为潜在的布鲁氏菌新型疫苗,有进一步研究的意义。 |
| 关 键 词： | 布鲁氏菌 17.3 ku 蛋白 基因疫苗 免疫应答 |
| 文章编号： | 1673-4696(2007)06-0505-05 |
| 修稿时间： | 2007年1月15日 |
Development of DNA vaccine pcDNA3.1-omp17.3 against Brucella |
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| DENG Xiao-hong,ZENG Zheng,WANG Xi-liang.Development of DNA vaccine pcDNA3.1-omp17.3 against Brucella[J].Veterinary Science in China,2007,37(6):505-509. | |
| Authors: | DENG Xiao-hong ZENG Zheng WANG Xi-liang |
| Abstract: | The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3.Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells,and the expressed OMP 17.3 was detected by Western-blotting.The recombinant plasmid was injected into BALB/c mice intramuscularly.The immunized mice were induced to produce a typical T helper 1 dominated immune response,as determined by ELISA,Flow Cytometer and ELISPOT method.The results showed that pcDNA3.1-omp17.3 was a potential candidate vaccine for the controlling of brucellosis. |
| Keywords: | Brucella 17 3 ku protein gene vaccine immune response |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |