血中十六种挥发性碳氢化合物的固相微萃取 |
| |
引用本文: | 梁克伟,刘俊亭,胡润平.血中十六种挥发性碳氢化合物的固相微萃取[J].中国法医学杂志,2001,16(4):218-220. |
| |
作者姓名: | 梁克伟 刘俊亭 胡润平 |
| |
作者单位: | 1. 中国医科大学基础医学院,辽宁,沈阳,110001 2. 阳泉市公安局,山西,阳泉,045100 |
| |
摘 要: | 为认定火灾现场烧死尸体 ,用固相微萃取技术从血中快速提取十六种碳氢化合物 (直链烃从八碳到十三碳 ,芳香烃从二甲苯异构体到三甲基苯 )。在室温条件下直接用 10 0 μm 聚二甲基硅氧烷萃取头以顶空方式萃取浓缩2 0min。气相色谱仪 ,氢火焰检测器测定 ,外标法定量。结果显示 :血中检测浓度可达 0 1μg/ml。固相微萃取是筛选血中碳氢化合物一种灵敏而准确的方法。
|
关 键 词: | 固相萃取 碳氢化合物 |
文章编号: | 1001-5728(2001)04-0218-03 |
修稿时间: | 2000年12月1日 |
Solid phase microextraction of 16 volatile hydrocarbons from blood |
| |
Abstract: | The present paper reports that 16 volatile hydrocarbons were extracted from blood by solid phase microextraction (SPME). Aliphatic hydrocarbons ranging from n Octane to n tridecane, and aromatic hydrocarbons ranging from m (o )xylene to trimethylbenzenes were used in this study. This method can be used for examining a burned body to ascertain whether the victim was alive or dead when the burning incident took place. The 16 volatile hydrocarbons were extracted for 20 min at room temperature (20 ℃) by a polydimethylsiloxane fiber. Gas chromatography was used for the analysis with flame ionization detection (FID). Each compound was detectable at least 0 1 μg/ml . This method is simple and accurate. In conclusion, this method is feasible for the screening of volatile hydrocarbons from blood in forensic medicine. |
| |
Keywords: | Solid phase microextraction Volatile hydrocarbons |
本文献已被 CNKI 万方数据 等数据库收录! |
|