| 高效液相色谱法测定人血清中安眠酮及其苯甲基羟化代谢物的浓度 |
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| 引用本文： | 褚秋珉,张爱琴,张其英.高效液相色谱法测定人血清中安眠酮及其苯甲基羟化代谢物的浓度[J].中国法医学杂志,1989(1). |
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| 作者姓名： | 褚秋珉 张爱琴 张其英 |
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| 作者单位： | 上海医科大学法医学教研室，上海医科大学法医学教研室，上海医科大学法医学教研室 |
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| 摘 要： | 本文建立了用反相高效液相色谱法(RP-HPLC)测定人血清中安眠酮及其苯甲基羟化代谢物-2-甲基-32-(羟甲基)-苯基]-4(3H)喹唑酮(Ⅰ)的浓度。安眠酮浓度在1-35μg/ml范围内呈直线相关(相关系数r=0.9980;回归方程y=0.06324x—0.1029),方法回收率平均为102.08±9.987(SD)％(n=5),检出限为1ng。其代谢物Ⅰ按原型安眠酮的线性浓度测定其相对量。本法为测定中毒者体内安眠酮及其代谢物Ⅰ的血浓度提供可行的手段。
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| 关 键 词： | 安眠酮 2-甲基-32-羟甲基-苯基]-43H喹唑酮 反相高效液相色谱法 |
Determination of methaqualone and 2-methyl-3[2-(hydroxymethyl) phenyl]-4 (3H)quinazolinone concentrations in human serum by RP-HPLC |
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| Chu Qiuming Zhang Aiqin Zhang Qiying.Determination of methaqualone and 2-methyl-3[2-(hydroxymethyl) phenyl]-4 (3H)quinazolinone concentrations in human serum by RP-HPLC[J].Chinese Journal of Forensic Medicine,1989(1). |
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| Authors: | Chu Qiuming Zhang Aiqin Zhang Qiying |
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| Institution: | Chu Qiuming Zhang Aiqin Zhang Qiying Department of Forensic Medince,Shanghai Medical University |
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| Abstract: | Methaqualone and 2-methyl-3 2- (hydroxymethyl) phenyl]- 4 (3H) quinazolinone (metabolite I) concentrations in human serum wereanalyzed quantitatively by reverse high performance liquid chromatography (RP-HPLC). The concentrations of methaqualone ranging from 1-35μg/ml were in good linear relationship(Correlation coefficient r = 0. 9980, regression line y = 0. 06324x-0. 1029), The average recovery of methaqualone reached as high as 102.08±9. 987(SD)% (n= 5). The detective limit was 1 ng. The relative quantity of metabolite I was determined according to the linear concentration of methaqulone. RP-HPLC assay is proved to be useful in determening blood methaqualone and metabolite I concentrations in forensic toxicology. |
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| Keywords: | RP-HPLC Methaqualone 2-methyl-3 [2-(hydroxymethyl) phenyl]-4(3H) quinaxolinone |
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