| 鸡传染性支气管炎病毒SC株S1基因的序列分析 |
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| 引用本文： | 刘洪义,刘兴友.鸡传染性支气管炎病毒SC株S1基因的序列分析[J].中国兽医科学,2003,33(12):47-50. |
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| 作者姓名： | 刘洪义 刘兴友 |
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| 作者单位： | 1. 西南科技大学,四川,绵阳,621000
2. 郑州牧业工程高等专科学校,河南,郑州,450011 |
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| 摘 要： | 用RT PCR扩增了鸡传染性支气管炎病毒 (IBV)SC株S1基因 ,连接到 pMD 18 T载体上 ,克隆后进行了核酸序列分析 ,证实SC株S1基因与基因库中收录的国外毒株H12 0和M 4 1的同源性较低 ,分别为 81.1%和 80 .0 % ,而与国内JX990 1株的同源性达到 91.3% ,初步证实国内存在IBV新毒株。
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| 关 键 词： | 鸡传染性支气管炎病毒 S1基因 核酸序列 |
| 文章编号： | 1000-6419(2003)12-0047-04 |
| 修稿时间： | 2003年3月25日 |
Sequence analysis of S1 gene of infectious bronchitis virus SC strain |
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| LIU Hong-yi,LIU Xing-you.Sequence analysis of S1 gene of infectious bronchitis virus SC strain[J].Veterinary Science in China,2003,33(12):47-50. |
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| Authors: | LIU Hong-yi LIU Xing-you |
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| Institution: | LIU Hong-yi~1,LIU Xing-you~2 |
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| Abstract: | S1 gene of infectious bronchitis virus (IBV) SC strain was amplified by RT-PCR and sequenced by automatic sequencing system. The sequence consisted of 1 737 bp. When S1 gene of SC strain was compared with that of H120, M41 or JX9901 strain, which have been published previously in GenBank, homologies between them were 81.1%, 80.0% or 91.3%, respectively. The results showed that the SC strain is a new IBV strain in China. |
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| Keywords: | infectious bronchitis virus S1 gene DNA sequence |
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