| NSP-ELISA鉴别FMDV感染与免疫 |
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| 引用本文： | 朱彩珠,张强,常惠芸,卢永干,邱孝高,尤永进,葛艳,陈波,饶忠,徐泉兴.NSP-ELISA鉴别FMDV感染与免疫[J].中国兽医科学,2003,33(8):3-6. |
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| 作者姓名： | 朱彩珠 张强 常惠芸 卢永干 邱孝高 尤永进 葛艳 陈波 饶忠 徐泉兴 |
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| 作者单位： | 1. 中国农业科学院,兰州兽医研究所,甘肃,兰州,730046
2. 上海市农业科学院,畜牧兽医研究所,上海,201106 |
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| 基金项目： | 上海市科学技术发展项目 (2 0 0 0 0 0 34130 0 9) |
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| 摘 要： | 利用大肠埃希氏菌表达的口蹄疫病毒 (FMDV)非结构蛋白 (NSP) 3ABC多肽作为抗原 ,建立了一种可区分FMDV感染与接种灭活疫苗免疫牛的间接酶联免疫吸附试验 (I ELISA)。通过对 336份正常牛血清、50 8份注射疫苗牛血清、60份人工感染牛血清和 58份豚鼠免疫血清的检测 ,确定了血清抗体阴性与阳性效价的临界值。试验证明 ,该方法简易、快速 ,灵敏度比VIAA AGID高
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| 关 键 词： | 口蹄疫病毒 非结构蛋白 间接酶联免疫吸附试验 |
| 文章编号： | 1000-6419(2003)08-0003-04 |
| 修稿时间： | 2003年4月24日 |
Differentiating infection from vaccination against foot-and-mouth disease by a non-structural protein ELISA |
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| ZHU Cai-zhu ,ZHANG Qiang ,CHANG Hui-yun ,LU Yong-gan ,QIU Xiao-gao ,YOU Yong-jin ,GE Yan ,CHEN Bo ,RAO Zhong ,X U Quan-xing.Differentiating infection from vaccination against foot-and-mouth disease by a non-structural protein ELISA[J].Veterinary Science in China,2003,33(8):3-6. |
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| Authors: | ZHU Cai-zhu ZHANG Qiang CHANG Hui-yun LU Yong-gan QIU Xiao-gao YOU Yong-jin GE Yan CHEN Bo RAO Zhong X U Quan-xing |
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| Institution: | ZHU Cai-zhu 1,ZHANG Qiang 1,CHANG Hui-yun 1,LU Yong-gan 1,QIU Xiao-gao 1,YOU Yong-jin 2,GE Yan 2,CHEN Bo 2,RAO Zhong 2,X U Quan-xing 2 |
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| Abstract: | An indirect ELISA was developed for differenti at ion of infection from vaccination against foot-and-mouth disease virus (FMDV) using a non-structural protein 3ABC as antigen expressed in Escherichia coli. The negative and positive cut off values for absorbances of the I -ELISA were established according to the results by detection of 962 serum samples, that included 336 sera from native cattles, 508 from vaccinated cattles , 60 from infected cattles, and 58 from hyperimmune cavians. The I-ELISA test i s easier to operate, quicker to obtain result, and more sensitive than the VIAA -AGID. |
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| Keywords: | foot-and-mouth disease virus non-structural prote in indirect-ELISA |
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