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| 大鼠血液、尿液中阿米替林的气相色谱快速分析 | |
| 引用本文： | 高丽伟,马祥涛,余立新,蔡向阳,刘缙,金鸣.大鼠血液、尿液中阿米替林的气相色谱快速分析[J].中国法医学杂志,2011,26(2):127-129. |
| 作者姓名： | 高丽伟 马祥涛 余立新 蔡向阳 刘缙 金鸣 |
| 作者单位： | 1. 华中科技大学同济医学院法医学系,湖北,武汉,430030;余姚市公安局,浙江,余姚,315400 2. 岳阳市公安局,湖南,岳阳,414000 3. 华中科技大学同济医学院法医学系,湖北,武汉,430030 |
| 摘 要： | 目的建专：m液及尿液中阿米替林（AMTL）的气相色谱分析方法，、方法以正常大鼠m液及尿液为空F1样奉，分别添加AMTI-标准品和内标SKF525A。实验大鼠以AMTL2倍LD50灌胃，致大鼠急性中毒后提取血液及尿液。用乙醚提取样本中AMTL，采用GC／FID法进行定量分析，并考察实验条件，结果采用该方法，血液及尿液中AMTL线性池用分别为5～150μg／mL（r=0．993）和5～150μg／mL（r=0．998）；最低检测限（S／N／〉3）均为1．0陆g／mL；口内、口间精密度均小于6％，同收率存95.5％～105．6％之间。结论该方法方操作便捷、捧确度高，适用=fAMTL临床治疗中血药浓度快速监测和法医毒物分析鉴定。 |
| 关 键 词： | 法医毒物分析 阿水替林 内标法 气相色谱法 |
Determination of amitriptyline in rat blood and urine by gas chromatography |
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| GAO Liwei,MA Xiangtao,YU Lixin,CAI Xiangyang,LIU Jin,JIN Ming.Determination of amitriptyline in rat blood and urine by gas chromatography[J].Chinese Journal of Forensic Medicine,2011,26(2):127-129. | |
| Authors: | GAO Liwei MA Xiangtao YU Lixin CAI Xiangyang LIU Jin JIN Ming |
| Institution: | (GAO Liwei1,2,MA Xiangtao1,YU Lixin3,CAI Xiangyang1,LIU Jin1,JIN Ming1/1.Faculty of Forensic Medicine,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030,China,2.Yuyao Municipal Public Security Bureau,Zhejiang Yuyao 315400,3.Yueyang Municipal Public Security Bureau,Hunan Yueyang 414000,China) |
| Abstract: | Objective To develop a method for the determination of amitriptyline in rat blood and urine by gas chromatography(GC).Methods The amitriptyline standard and internal standard was added to blank samples of blood and urine.The experimental rats were given amitriptyline in double dosage of the LD50 with intragastric administration.Blood and urine were extracted from poisoned rats.Amitriptyline was extracted from samples with diethyl ether and for quantitative analysis by GC/FID.Experimental conditions were stu... |
| Keywords: | forensic toxicology analysis amitriptyline internal standard gas chromatography |
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