| 广东地区汉族人群 13个 STR基因座的频率调查 |
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| 作者姓名： | Li Y Wang SB Liu C Li HX Hu HY Liu H Liu CH Chen XH |
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| 作者单位： | 广州市刑事科学技术研究所, |
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| 基金项目： | 广东省科技攻关项目,99M04813G, |
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| 摘 要： | 目的调查广东地区汉族无关个体的 13个 STR基因座（ D3S1358、 vWA、 FGA、 D8S1179、 D21S11、 D18S51、 D5S818、 D13S317、 D7S820、 D1 6S539、 TH01、 TPOX、 CSF1PO）多态性,研究其在法医学检验中的应用价值。方法用 AmpFlSTR Profiler Plus及 Cofiler二个荧光标记系统对新鲜血样进行 13个基因座的复合扩增,用 ABI 377-96全自动测序仪对扩增产物进行检测,用 GenoTyper软件进行基因分型。结果 13个基因座 PIC >0.5,DP >0.76,家系调查符合孟德尔遗传规律。结论含有 13个 STR基因座的二个荧光标记复合扩增系统可满足法医物证学的个体识别及亲权鉴定的需要。
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| 关 键 词： | 复合扩增 荧光标记 STR基因座 个体识别 亲权鉴定 |
| 文章编号： | 1004-5619(2001)02-0082-04 |
| 修稿时间： | 2000年9月26日 |
Genetic studies of 13 loci in Guangdong Han population by two multiplex PCR systems and its forensic application |
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| Li Y,Wang SB,Liu C,Li HX,Hu HY,Liu H,Liu CH,Chen XH.Genetic studies of 13 loci in Guangdong Han population by two multiplex PCR systems and its forensic application[J].Journal of Forensic Medicine,2001,17(2):82-85. |
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| Authors: | Li Y Wang S B Liu C Li H X Hu H Y Liu H Liu C H Chen X H |
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| Institution: | Guangzhou Criminal Science & Technology Institute, Guangzhou 510030. |
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| Abstract: | OBJECTIVE: The genetic studies of 13 short tandem repeats(STRs) loci in two multiplex amplification systems were carried out on Chinese Han population in Guangdong. METHODS: DNA samples from 328 unrelated individuals were screened. The 13 loci were D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, TH01, TPOX and CSF1PO. The PCR products were analyzed and genotyped by ABI 377-96 Sequencer. RESULTS: The combined power of discrimination (DP) was 0.999999999999993 and the combined paternity of exclusion(PE) was 99.999%. These 13 loci met the Hardy-Weinberg expectations. CONCLUSION: The two multiplex amplification systems were very useful in forensic case investigation. |
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| Keywords: | multiplexing PCR STR loci forensic science |
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