| 高效液相色谱法检测人脊髓中的利多卡因 |
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| 作者姓名： | Jing M Wu G Yang D |
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| 作者单位： | 1. 同济医科大学法医学系化学教研室,湖北武汉,430030
2. 同济医科大学实验医学研究中心,湖北武汉,430030 |
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| 摘 要： | 建立高效液相色谱法对人脊髓中利多卡因进行分析的方法 ,以扩大药 (毒 )物检测范围及检测手段 ,适应法医学鉴定及对特殊检材检验的需要。用利多卡因标准品及空白脊髓标准添加实验 ,对色谱条件、样品处理方法、回收率及方法的线性和精密度进行了系统考察。所建方法线性范围是2 0～20 0μg/ml(r=0 9999) ,最低检测限为0 2μg/ml(S/N≥3),加样回收率为82 4 %～92 7% ,该法选择性好、不受干扰。所建方法灵敏、准确、简捷 ,可用于法医学鉴定。
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| 关 键 词： | 利多卡因 高效液相色谱 脊髓 |
| 修稿时间： | 2000－05－08) |
Determination of lidocaine in human spinal cord by high-performance liquid chromatography |
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| Jing M,Wu G,Yang D.Determination of lidocaine in human spinal cord by high-performance liquid chromatography[J].Journal of Forensic Medicine,2000,16(3):153-154. |
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| Authors: | Jing M Wu G Yang D |
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| Institution: | Faculty of Forensic Medicine, Tongji Medical University, Wuhan 430030, PRC. |
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| Abstract: | A RPHPLC method for determining lidocaine in human's spinal cord. Was established. The linear range of the method was 0.20-20.0 micrograms/ml(r = 0.9999). The detection limit was 0.02 ng/ml(S/N > or = 3) and the clean-up recovery of lidocaine in human spinal cord was 82.4%-92.7%. The CV% were no more than 6.0% (N = 4). No interfere by other compounds was observed in study results using this method. RPHPLC method shows an excellent recovery, high sensitivity and good precision. |
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| Keywords: | Lidocaine HPLC Humanspinalcord |
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