| Institution: | 1. California National Primate Research Center, University of California, Davis, CA 95616.;2. Department of Anthropology, University of California, Davis, CA 95616.;3. Graduate Group in Forensic Science, University of California, Davis, CA 95616.;4. Finnzymes Diagnostics, Keilaranta 16 A, 02150 Espoo, Finland.;5. MMI Genomics, Inc., 1756 Picasso Avenue, Davis, CA 95618.;6. The Jan Bashinski DNA Laboratory, California Department of Justice, Richmond, CA 94804.;7. QuestGen Forensics, 1046 Olive Drive Suite 1, Davis, CA 95616.;8. Department of Forensic and Investigative Genetics, University of North Texas Health Science Center, Ft. Worth, TX 76107.;9. Institute of Investigative Genetics, University of North Texas Health Science Center, Ft. Worth, TX 76107. |
| Abstract: | Abstract: Canine biological specimens are often part of the physical evidence from crime scenes. Until now, there have been no validated canine‐specific forensic reagent kits available. A multiplex genotyping system, comprising 18 short tandem repeats (STRs) and a sex‐linked zinc finger locus for gender determination, was developed for generating population genetic data assessing the weight of canine forensic DNA profiles. Allele frequencies were estimated for 236 pedigreed and 431 mixed breed dogs residing in the U.S. Average random match probability is 1 in 2 × 1033 using the regional database and 1 in 4 × 1039 using the breed dataset. Each pedigreed population was genetically distinct and could be differentiated from the mixed breed dog population but genetic variation was not significantly correlated with geographic transition. Results herein support the use of the allele frequency data with the canine STR multiplex for conveying the significance of identity testing for forensic casework, parentage testing, and breed assignments. |
