| 4个多拷贝RM Y-STR基因座的异常分型 |
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| 引用本文： | 张文琼,肖超,余谨,贾雲舒,黄代新.4个多拷贝RM Y-STR基因座的异常分型[J].中国法医学杂志,2016(5):476-479. |
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| 作者姓名： | 张文琼 肖超 余谨 贾雲舒 黄代新 |
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| 作者单位： | 1. 华中科技大学同济医学院法医学系，湖北武汉430030; 青岛市李沧区人民检察院，山东青岛266100;2. 华中科技大学同济医学院法医学系,湖北武汉,430030;3. 武汉血液中心，湖北武汉，430030 |
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| 摘 要： | 目的观察湖北汉族人群中4个多拷贝RM Y-STR基因座的异常分型。方法使用文献报道及自行设计的荧光标记引物对252个无关男性个体样本进行扩增,并用AB 3130遗传分析仪对扩增产物进行检测分析。结果在所有样本中共发现25例异常多等位基因分型,其中基因座DYF387S1出现5例,DYF399S1出现15例,DYF403S1出现1例,DYF404S1出现4例。25例异常分型存在于20个样本中,发生率为7.94%,其中4个样本在两个以上基因座上均出现异常分型。结论多拷贝RM Y-STR基因座异常分型发生率较高,在法医学的实际应用中应予以注意。
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| 关 键 词： | 法医物证学 Y-STR 快速突变 异常基因型 |
Anomalous genotype observation of 4 multi-copy RM Y-STRs |
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| Abstract: | Objective To observe anomalous genotypes of 4 multi-copy RM Y-STRs in Han population in Hubei province. Methods 252 unrelated male samples were ampliifed using reported and newly designed primers, then detected and analyzed by AB 3130 genetic analyzer. Results A total of 25 anomalous multi-band patterns were observed in 20 samples corresponding to an incident rate of 7.94%. 5 anomalous genotypes were observed in DYF387S1 locus, 15 in DYF399S1, 1 in DYF403S1 and 4 in DYF404S1. Four samples showed extra alleles in more than one locus. Conclusion Anomalous genotype has high incident rates in RM Y-STR markers and requires extensive attention in forensic practice. |
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| Keywords: | forensic biological evidence Y-STR rapidly mutating anomalous genotype |
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