MHC B-L基因多态性与鸡白痢沙门氏菌易感性的关联分析

为研究MHC B-L基因的多态性与鸡白痢沙门氏菌感染之间的关系,采用PCR产物直接测序技术对供试鸡MHC B-L基因外显子2进行单核苷酸多态性检测,分别利用Plink1.07软件和PHASE V2.1软件进行病例-对照关联分析和单倍型分析。结果发现,在270bp的序列内共检测到42个单核苷酸多态性位点(SNPs)。C.280C>A位点等位基因在病例-对照样本中的分布存在显著差异(P<0.05);C.246G>T、C.249A>G位点基因型在病例-对照样本中的分布存在显著差异(P<0.05)。C.229T>A和C.249A>G位点组成的单倍型与抗鸡白痢性状差异不显著(P>0.05);同时C.285G>A、C.287A>T和C.288C>A位点组成的单倍型与抗鸡白痢性状的差异也不显著(P>0.05)。结果表明,MHC B-L基因外显子2多态性与抗鸡白痢性状显著相关,为开展沙门氏菌抗性分子标记辅助选择研究提供了一定的依据。

Association of MHC B-L gene polymorphism with susceptibility of Salmonella pullorum

To study the association between MHC B-L gene polymorphism and Salmonella pullorum infection,polymerase chain reaction sequence-based typing method was used to detect the polymorphism of MHC B-L gene exon 2.The Plink1.07 and PHASE V2.1 software was used as case-control association study and haplotype analysis,respectively.There were 42 single nucleotide polymorphisms(SNPs) in 270 bp DNA sequence.The distribution of C.280C>A alleles had a significant difference in case-control samples.The distribution of C.246G>T and C.249A>G genotypes had a significant difference in case-control samples.Haplotypes within the C.229T>A and C.249A>G sites and within the C.285G>A,C.287A>T and C.288C>A sites did not associate with anti-pullorum disease trait.The results showed that the polymorphism of MHC B-L gene exon 2 was significant associated with anti-pullorum disease trait,and provi-ded some bases for developing marker assisted selection programmes for anti-salmonellosis.