| 牛源犬新孢子虫MAG1基因的克隆及原核表达 |
| |
| 引用本文： | 焦石,贾立军,薛书江,刘明明,黄国明,张守发.牛源犬新孢子虫MAG1基因的克隆及原核表达[J].中国兽医科学,2012(1):31-34. |
| |
| 作者姓名： | 焦石 贾立军 薛书江 刘明明 黄国明 张守发 |
| |
| 作者单位： | 延边大学农学院动物医学系 |
| |
| 基金项目： | 吉林省自然科学基金项目(201115230) |
| |
| 摘 要： | 为了解牛源犬新孢子虫MAG1基因的免疫学特性,根据MAG1基因序列,设计并合成了1对用于扩增MAG1基因的引物。将PCR扩增产物连接到原核表达载体pGEX-4T-2上,转化大肠杆菌BL21(DE3)中。SDS-PAGE结果显示,MAG1在大肠杆菌中获得了较高水平的表达,表达蛋白的分子质量为65ku。Western-blot结果显示,表达的MAG1蛋白可被牛源犬新孢子虫阳性血清特异性识别,表明该MAG1蛋白具有较好的反应原性。本研究为新孢子虫病疫苗及诊断试剂盒的研究奠定了基础。
|
| 关 键 词： | 犬新孢子虫 MAG1基因 原核表达 |
Cloning and prokaryotic expression of MAG1 gene of bovine Neospora caninum |
| |
| JIAO Shi,JIA Li-jun,XUE Shu-jiang,LIU Ming-ming,HUANG Guo-ming,ZHANG Shou-fa.Cloning and prokaryotic expression of MAG1 gene of bovine Neospora caninum[J].Veterinary Science in China,2012(1):31-34. |
| |
| Authors: | JIAO Shi JIA Li-jun XUE Shu-jiang LIU Ming-ming HUANG Guo-ming ZHANG Shou-fa |
| |
| Institution: | (Department of Veterinary Medicine,Agricultural College,Yanbian University,Yanji 133002,China) |
| |
| Abstract: | To understand immunological characteristics of MAG1 gene of bovine Neospora caninum,a pair of primers for MAG1 gene was designed according to the sequence of MAG1 gene.The MAG1 gene was amplified,and cloned into the pGEX-4T-2 vector and expressed in Escherichia coli BL21(DE3).SDS-PAGE analysis result indicated that MAG1 could be expressed high level in E.coli,the molecular weight of expressed protein was 65 ku.Western-blot analysis showed that the expressed MAG1 protein could specifi-cally react with the positive serum against bovine N.caninum,indicating the protein had good reactinogenicity.The present study provided the foundation for the development of vaccines and diagnostic kits of neosporaosis. |
| |
| Keywords: | Neospora caninum MAG1 gene prokaryotic expression |
| 本文献已被 CNKI 等数据库收录！ |
|
