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| 猪源新城疫病毒的分离鉴定 | |
| 引用本文： | 程龙飞,柯美峰,郑腾,黄瑜,李文杨,施少华.猪源新城疫病毒的分离鉴定[J].中国兽医科学,2004,34(12):66-68. |
| 作者姓名： | 程龙飞 柯美峰 郑腾 黄瑜 李文杨 施少华 |
| 作者单位： | 1. 福建省农业科学院,畜牧兽医研究所,福建,福州,350013 2. 福州大北农生物技术有限公司,福建,福州,350014 3. 福建出入境检验检疫局,福建,福州,350003 |
| 摘 要： | 从发生呼吸道症状的病猪鼻腔中分离到 1株病毒 ,经电镜观察、血凝和血凝抑制试验 ,确定为新城疫病毒。经对鸡胚平均致死时间、鸡胚半数致死量、对鸭胚和鸡、鸭的致死性测定及荧光PCR检测 ,证明分离毒为温和型毒株。 |
| 关 键 词： | 新城疫病毒 猪 分离鉴定 |
| 文章编号： | 1000-6419(2004)12-0066-03 |
| 修稿时间： | 2004年7月1日 |
Isolation and identification of Newcastle disease virus from swine |
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| CHENG Long-fei,KE Mei-feng,ZHENG Teng,HUANG Yu,LI Wen-yang,SHI Shao-hua.Isolation and identification of Newcastle disease virus from swine[J].Veterinary Science in China,2004,34(12):66-68. | |
| Authors: | CHENG Long-fei KE Mei-feng ZHENG Teng HUANG Yu LI Wen-yang SHI Shao-hua |
| Institution: | CHENG Long-fei~1,KE Mei-feng~2,ZHENG Teng~3,HUANG Yu~1,LI Wen-yang~1,SHI Shao-hua~1 |
| Abstract: | A strain of virus was isolated from cavum nasi of swine with respiratory symptoms and identified to be Newcastle disease virus by electro-microscope observation, hemagglutination test and hemagglutination inhibition test. The isolated virus was proved to be a strain of moderate virus by (detecting) its mean death time to chicken embryo, LD_(50) to SPF chicken embryo, lethality to duck embryos, chickens and ducks, and by fluorescent PCR detection. |
| Keywords: | swine Newcastle disease virus isolation and identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |