猪水泡病病毒结构蛋白编码区核苷酸的序列测定与分析

以猪水泡病病毒 (Swinevesiculardiseasevirus ,SVDV)HK’70为材料 ,用特异性引物扩增出P1区的 2个重叠目的片段 ,连接于pMD 18 T载体 ,转化JM 10 9感受态细胞。经重组质粒的双酶切 ,PCR鉴定后测序。序列分析表明 ,HK’70P1区核苷酸组成中A含量较高 ,G C含量较低 ,且A G、C T转换率较高。P1序列同源性分析表明 ,SVDVHK’70与J1’73、H/3’76、SVDV(Seechurn等测株 )、NET/1/92的核苷酸序列同源性分别为 97.8%、98.1%、97.6 %和 89.3% ;相应地 ,推导的氨基酸序列同源性分别为 98.8%、98.7%、98.8%和 96 .5 %。

Determination and analysis of nucleotide sequence encoding structure protein of swine vesicular disease virus

Two fragments of overlapping SVDV P1 genome were amplified by the specific primers, then ligated with pMD 18-T vector and transformed into JM109. The recombinant plasmids were identified by restriction enzyme and the PCR. P1 sequence analysis indicated that adenosine(A) was rich, guanosine(G) and cytidine (C) were poor and A-G,C-T changing rates were high. Homology analysis showed that P1 sequence of SVDV HK'70 shared 97.8%,98.1%,97.6% and 89.3% homology with that of SVDV J1'73, SVDV H/3'76, SVDV (Seechurn) and SVDV NET/1/92 respectively, and the deduced amino acids sequence from P1 sequence of SVDV HK'70 shared 98.8%,98.7%,98.8% and 96.5% homology with that from P1 sequence of SVDV J1'73, SVDV H/3'76, SVDV(Seechurn) and SVDV NET/1/92 (respectively.)