应用新城疫病毒强弱毒株RT-PCR鉴别诊断技术检测多种禽类副黏病毒

应用已建立的新城疫病毒 (NDV)强、弱毒株RT PCR快速鉴别诊断技术 ,对来自广西各地疑为禽副黏病毒血清 1型 (APMV 1)感染的 14 4份不同禽类的病料进行了检测 ,并用常规方法对RT PCR检测为阳性的部分病料进行了病毒的分离和鉴定。结果 ,从鸡、鸽、鹅、鸭、鹌鹑、珍珠鸡、孔雀、鸵鸟和画眉鸟 9种禽的病料样品中检测到APMV 1,阳性检出率为 6 4.5 8% (93/ 14 4 ) ;从 7种禽病料中分离到 2 9株APMV 1地方野毒株。部分分离株用NDV强、弱毒株快速鉴别技术鉴定属中、强毒株。

Application of the rapid differential diagnosis technique for Newcastle disease to detection of avian paramyxoviruses

The rapid differential diagnosis technique for detection of virulent and non-virulent NDVs was used to detect 144 clinical samples of avian paramyxovirus type 1 (APMV-1) suspect from different poultry species. Some positive samples were also diagnosed by the classical isolation and identification. The results showed that 64.58% of the samples from chickens, pigeons, geese, ducks, quail, peacock, thrush and ostrich respectively were positive of NDV. 29 Field strains of APMV-1 were isolated from 7 (avian) species origins and some were classified as mesogenic or velogenic strains of NDV by the rapid differential diagnosis technique. The results demonstrated that the developed technique of RT-PCR basis is a specific, sensitive, rapid and reliable for the diagnosis of APMV-1 infections in different poultry species origins and applicable in the laboratory diagnosis.