应用PCR检测人工感染鸡体内鸡传染性贫血病毒的动态分布

应用聚合酶链反应（ＰＣＲ）技术检测人工感染１２周龄ＳＰＦ鸡体内鸡传染性贫血病毒（ＣＡＶ）的动态分布结果，感染后第２周可从外周血液白细胞中检出ＣＡＶＤＮＡ，在第３周时达到最高峰，然后逐渐下降，第６周时检出率仅为６．３％（３／４８），第７周以后均未能从血液白细胞中检测到ＣＡＶＤＮＡ；人工感染后第２～１２周均能从骨髓、胸腺、脾、肾、肝和腔上囊等组织检出ＣＡＶＤＮＡ，其中以骨髓和胸腺的检出率最高（８５．０％），其次是脾（８０．０％）、肾和肝（６０．０％），腔上囊最低（２５．０％），从１２周以后上述几种组织中再未检测到ＣＡＶＤＮＡ。并从ＰＣＲ检测结果为阳性的组织中也分离到ＣＡＶ。

Dynamic distributions of chicken anemia virus in the artificially infected chicks by PCR

A polymerase chain reaction (PCR) technique was used to determine chicken anemia virus (CAV) in 12 week old SPF chicks artificially infected with CAV. The test results revealed that the CAV DNA were decected in the leucocytes of the peripheral blood from the tested fowls week 2 after the CAV artificial infection and the detectable rate reached a peak value weed 3 after the infection and then on fell gradually and the detectable rate only was 6.7%(3/48) week 6 after the infection and no CAV DNA in the leucocytes was detected week 7 after the infection. And CAV DNA all were detected in the bone marraws, thymus, spleens, kidneys and the bursa of Fabricius from the chicks infected artificially with CAV weeks 2 to 12 after the infection, out of which the detectable rates of bone marrow and thymus were highest (85%), the next was spleen (80%), the liver and kidney (60%), the lowest was bursa of the Fabricius (25%). No CAV DNA was detected in the above several organs after 12 weeks of the infection.