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| 应用PCR-SSCP技术检测PGM1基因型 | |
| 引用本文： | Song HY,Yang QE,Yu CY. 应用PCR-SSCP技术检测PGM1基因型[J]. 法医学杂志, 2002, 18(3): 152-154 |
| 作者姓名： | Song HY Yang QE Yu CY |
| 作者单位： | 华中科技大学同济医学院法医学系,湖北,武汉,430030 |
| 摘 要： | 目的应用PCR-SSCP技术分型PGM1基因型.方法提取156份武汉地区汉族无关个体的血样DNA,分别扩增PGM1基因外显子4和外显子8的多态性靶DNA,用SSCP分析PCR产物,判断基因型.结果两种PCR产物均检出了两个等位基因、三种基因型,DP值分别为0.5620、0.4405.综合外显子4和8的PCR-SSCP结果,分出8种PGM 1基因型,DP值为0.731 8.应用本法对保存10年的陈旧血痕和精斑PGM1分型成功.结论用PCR-SSCP分型PGM1基因型在法医物证检验中具有实用价值. |
| 关 键 词： | 磷酸葡萄糖变位酶1(PGM1) 基因型 PCR-SSCP |
| 文章编号： | 1004-5619(2002)03-0152-03 |
| 修稿时间： | 2002-01-08 |
PGM1 genotyping by PCR-SSCP |
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| Song Hai-yan,Yang Qing-en,Yu Chun-ying. PGM1 genotyping by PCR-SSCP[J]. Journal of Forensic Medicine, 2002, 18(3): 152-154 | |
| Authors: | Song Hai-yan Yang Qing-en Yu Chun-ying |
| Affiliation: | Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030. |
| Abstract: | Objective PGM 1 genotyping by PCR-SSCP analysis.Methods Amplified genome DNA from156unrelated Han individuals living in Wuhan,PCR products for exon4and exon8of PGM 1 were then analyzed by SSCP to detect the genotypes.Results2alleles and3genotypes were detected in exon4and8respectively.The discrimination power was0.7318.PCR-SSCP analysis was suitable for determination of PGM 1 genotypes from old blood and semen stains.Conlusion PGM 1 system typed by PCR-SSCP is useful for forensic identification. |
| Keywords: | PGM 1 genotype PCR-SSCP |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |