| 应用分子印迹固相萃取法提取血液中大隆和溴敌隆 |
| |
| 引用本文： | 常靖,郝红霞,侯小平,张云峰,栾玉静.应用分子印迹固相萃取法提取血液中大隆和溴敌隆[J].中国法医学杂志,2011,26(5):371-373,376. |
| |
| 作者姓名： | 常靖 郝红霞 侯小平 张云峰 栾玉静 |
| |
| 作者单位： | 1. 公安部物证鉴定中心,北京,100038
2. 证据科学教育部重点实验室 中国政法大学,北京,100088 |
| |
| 摘 要： | 目的采用分子印迹固相萃取,UPLC/MS分析,检验血液中大隆和溴敌隆。方法以大隆为模板分子,丙烯酰胺为单体,二甲基亚砜为有机溶剂,按照模板分子与单体摩尔配比关系为1∶4合成大隆分子印迹聚合物,作为固相萃取剂萃取血液中大隆和溴敌隆,以2×1mL去离子水、1mL 30%甲醇去离子水洗涤杂质,2×1mL 1%甲酸/甲醇洗脱,提取血中目标物后进行UPLC/MS分析检测。结果溴敌隆和大隆回收率均在90%以上,在50～500ng/mL浓度范围内线性关系良好r,2溴敌隆为0.998 4,大隆为0.991,检测限（S/N=5/1）低于10ng/mL。结论本方法回收率高,基质效应降低,杂质干扰少,可用于血液中低浓度大隆和溴敌隆的检验。
|
| 关 键 词： | 法医毒物分析 大隆 溴敌隆 分子印迹固相萃取 UPLC/MS |
Aplication of molecularly imprinted solid phase extraction in separating and assaying anticoagulant raticide in blood samples |
| |
| CHANG Jing,HAO Hongxia,HOU Xiaoping,ZHANG Yunfeng,LUAN Yujing.Aplication of molecularly imprinted solid phase extraction in separating and assaying anticoagulant raticide in blood samples[J].Chinese Journal of Forensic Medicine,2011,26(5):371-373,376. |
| |
| Authors: | CHANG Jing HAO Hongxia HOU Xiaoping ZHANG Yunfeng LUAN Yujing |
| |
| Institution: | (CHANG Jing1,HAO Hongxia2,HOU Xiaoping1,ZHANG Yunfeng1,LUAN Yujing1/1.Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China,2.Key Laboratory of Evidence Science(CUPL),Ministry of Education,Beijing 100088,China) |
| |
| Abstract: | Objecive Molecular imprinted polymer are used as solid-phase extraction agents to extract brodifacoum and bromoadiolone from blood samples.Methods The brodifacoum imprinted molecule is synthesized with the ratio of 1mol brodifacoum template molecule to 4 mol acrylamide monomer molar.The solid-phase extraction(SPE) procedure is suggested as: elute interferences are washed out with 1 mL DI water twice and 1 ml 30% MeOH once respectively;brodifacoum and bromoadiolone are eluted with 1 mL 1% formic acid in meth... |
| |
| Keywords: | forensic toxicology analysis brodifacoum bromoadiolone molecularly imprinted solid phase extraction UPLC/MS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
