A X-chromosome STR hexaplex as a powerful tool in deficiency paternity cases |
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Authors: | Juliana Aquino Carla Peixe Dayse Silva Celso Tavares Elizeu F de Carvalho |
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Institution: | aDNA Diagnostic Laboratory (LDD), Biology Institute, State University of Rio de Janeiro, Brazil;bPathology Department, Faculty of Medicine, State University of Rio de Janeiro, Brazil |
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Abstract: | The X-chromosome short tandem repeat (STR) markers have been described as very adequate tools for solving deficiency paternity cases and kinship tests when women are involved. In the absence of the alleged father, presumed paternal relationship can be more efficiently investigated by using a set of six to ten X-STR markers compared to fifteen autosomal STR. For this study, we compared the usefulness of a X-STR hexaplex developed in our laboratory (DXS7133, DXS7424, DXS8378, DXS6807, DXS7423 and DXS8377) and the commercial kit Identifiler in solving deficiency paternities. We have worked on distinct groups of caseworks involving daughters, their mothers and presumed paternal grandmothers or putative half sisters and their respective mothers. The PCR products were separated by capillary electrophoresis and detected in an ABI Prism 3100. In the majority of the caseworks (>90%), the likelihood ratio (LR) obtained by using the X-STR hexaplex was higher than the LR value observed when the Identifiler kit was used for genotyping. The combination of the two STR typing systems was able to solve all the cases. |
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Keywords: | X-chromosome STR markers Paternity Deficiency cases Likelihood ratio Women |
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