| 用酶标抗人精液单克隆抗体A_(10)C_6建立ELISA斑点法快速鉴定人精(液)斑 |
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| 引用本文： | 李玉祥,余伯建,陈和军,李官成.用酶标抗人精液单克隆抗体A_(10)C_6建立ELISA斑点法快速鉴定人精(液)斑[J].中国法医学杂志,1995(1). |
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| 作者姓名： | 李玉祥 余伯建 陈和军 李官成 |
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| 作者单位： | 湖南省公安厅刑科所!长沙，410001，湖南省公安厅刑科所!长沙，410001，湖南省公安厅刑科所!长沙，410001，湖南医科大学肿瘤研究所!长沙，410078 |
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| 摘 要： | 作者用过氧化物酶标记自己研制的抗人精液单克隆抗体A10C6建立了简便快速鉴定人精斑的ELISA斑点法，直接通过酶标抗体上的酶催化底物显示颜色变化，来判断结果，阳性呈灰色斑点，阴性为无色。其结果只对人精斑浸出液显示阳性斑点，对人的其他组织器官及体液均呈阴性，对动物精斑也无交叉反应，精斑浸出液稀释到1：3200倍仍呈阳性结果。
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| 关 键 词： | 酶标A_（10）C_6 单克隆抗体 斑点法 人精斑 |
ESTABLISHMENT OF A RAPID DOT-ELISA METHED FOR IDENTIFYING HUMAN SEMINAL STAIN BY USING ENZYME LABLED ANTI-HUMAN SEMEN MONOCLONAL ANTIBODY(A_（10）C_6) |
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| Li Yuxiang, Yu Bojian, Chen Hejun.ESTABLISHMENT OF A RAPID DOT-ELISA METHED FOR IDENTIFYING HUMAN SEMINAL STAIN BY USING ENZYME LABLED ANTI-HUMAN SEMEN MONOCLONAL ANTIBODY(A_（10）C_6)[J].Chinese Journal of Forensic Medicine,1995(1). |
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| Authors: | Li Yuxiang Yu Bojian Chen Hejun |
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| Abstract: | A simple rapid and sensitive Dot-ELISA method was establishhed for identifying the human seminal stains by using anti-human semen monoclonal antibody (A10C6)labeled with horseradish Peroxidase of grayish color revealed a positive reaction. The results showed that the extract of human semen was positive. No Cross-reaction was observed when human tissues body fluids and animal seminal stains were tested. |
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| Keywords: | Enzyme labeled A_(10)C_6 Monoclonal antibody Dot-Elisa Seminal stain |
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