| 牛传染性鼻气管炎弱毒活疫苗的安全性和免疫保护效果 |
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| 引用本文： | 郭利,张淑琴,王炜,冷雪,武华. 牛传染性鼻气管炎弱毒活疫苗的安全性和免疫保护效果[J]. 中国兽医科学, 2012, 0(4): 380-383 |
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| 作者姓名： | 郭利 张淑琴 王炜 冷雪 武华 |
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| 作者单位： | 中国农业科学院特产研究所 |
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| 基金项目： | 牛羊重大疫病防控技术研究与产业化项目(200803018) |
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| 摘 要： | 为评价牛传染性鼻气管炎弱毒株的安全性及其免疫保护效果,将牛传染性鼻气管炎病毒(IBRV)驯化培养,接种MDBK细胞,优化病毒增殖条件,并将其免疫牛,评价其安全性及免疫保护效果。结果显示,优化后的病毒效价可达109.0 TCID50/mL。动物试验结果显示,3月龄牛接种该弱毒株后,体温正常,无任何临床异常表现。对免疫牛进行的攻毒试验结果显示,此弱毒株对牛的免疫保护效果良好。上述结果表明,该毒株可以作为研制弱毒活疫苗的候选毒株。
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| 关 键 词： | 牛传染性鼻气管炎病毒 弱毒活疫苗 安全性 免疫保护效果 |
Safety and protective efficacy of a live attenuated vaccine against bovine rhinotracheitis virus |
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| GUO Li,ZHANG Shu-qin,WANG Wei,LENG Xue,WU Hua. Safety and protective efficacy of a live attenuated vaccine against bovine rhinotracheitis virus[J]. Chinese Veterinary Science, 2012, 0(4): 380-383 |
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| Authors: | GUO Li ZHANG Shu-qin WANG Wei LENG Xue WU Hua |
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| Affiliation: | (Institute of Special Wild Economic Animal and Plant Science,Chinese Academy of Agricultural Sciences,Jilin 132109,China) |
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| Abstract: | The infectious bovine rhinotracheitis virus(IBRV) was habitually culture with MDBK cells that was preserved in our laboratory.The high virus titer of 108.7TCID50/mL was achieved by optimized replicating conditions.Safety and protective efficiency of the attenuated vaccine was tested in host animals.The evaluation results showed that 3-months-calves vaccinated with the attenuated vaccine did not show any clinical disease and the body temperature was normal.Immunogenicity study demonstrated that the strain has good protection to calves against IBRV-LN01/08 infection and clinical diseases caused by the challenge.Therefore,the attenuated strain can used as the ideal vaccine candidate strain against IBRV. |
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| Keywords: | infectious bovine rhinotracheitis virus(IBRV) immunization safety protective efficacy |
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