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| SPE-GC／MS法检测大鼠尿中尼美西泮及其代谢物 | |
| 引用本文： | 黄克建,LI Hong-sen,潘智文,林翠梧,刘晓锋,罗正坚,李璐,伍文坚.SPE-GC／MS法检测大鼠尿中尼美西泮及其代谢物[J].中国法医学杂志,2008,23(4):238-240. |
| 作者姓名： | 黄克建 LI Hong-sen 潘智文 林翠梧 刘晓锋 罗正坚 李璐 伍文坚 |
| 作者单位： | 1. 广西壮族自治区公安厅物证鉴定中心,广西,南宁,530012 2. 广西大学化学化工学院,广西,南宁,530004 3. 南宁市公安局禁毒支队,广西,南宁,530004 |
| 摘 要： | 目的检测尼美西痒阳性大鼠尿液中的代谢物。方法2只Wistar大鼠分别喂食半粒尼美西泮药丸，收集24h内尿液，用B葡萄糖醛酸酶水解，Oasis@HLB柱固相提取，以DB．35Ms柱为分离柱，气相色谱质谱联用法检测。结果从大鼠尿液中检出尼美西泮的4种代谢物：7-乙酰氨基尼美西泮、7-乙酰氨基硝基安定、7-氨基尼美西泮和2-氨基-5-硝基苯基苯甲酮及少量尼美西泮原体。结论尼美西泮在大鼠体内易代谢，在尿液中的主要代谢物为7-乙酰氨基尼美西泮和7-乙酰氨基硝基安定。 |
| 关 键 词： | 法医毒物分析 尿液 代谢物 尼美西泮 固相提取 气相色谱质谱 |
Determination of nimetazepam and its metabolites in rat urine by SPE-GC/MS |
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| LI Hong-sen.Determination of nimetazepam and its metabolites in rat urine by SPE-GC/MS[J].Chinese Journal of Forensic Medicine,2008,23(4):238-240. | |
| Authors: | LI Hong-sen |
| Institution: | HUANG Ke-jian, LI Hong-sen, PAN Zhi-wen, et al.( Alnstitue of Forensic Science, Public Security of Guangxi Province, Nanning 530012, China) |
| Abstract: | Objective To determine the metabolites of nimetazepam in rat.Methods Two Wistar rats were administered a pill of nimetazepam respectively,and urine fractions in 24 h were collected.After enzymatic hydrolysis of the urine samples,nimetazepam and its metabolites were extracted by Oasis HLB solid-phase extraction and analyzed by GC/MS with DB-35 MS column.Results Four metabolites for nimetazepam as 7-acetylaminonimetazepam,7-acetylaminonitrazepam,7-aminonimetazepam and 2-amino-5-nitrophenylphenylmethanone and a little nimetazepam were identified in the urine.Conclusion Nimetazepam was metabolized easily in rat and 7-acetylaminonimetazepam and 7-acetylaminonitrazepam were the main metabolites in rat urine. |
| Keywords: | Forensic toxicology analysis Urine Metabolite Nimetazepam Solid phase extraction Gas chromatography and mass spectrometry |
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