| SPE-HPLC分析全血中37种药（毒）物 |
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| 作者姓名： | Liang C Zhang YR Jin QY Guo YM |
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| 作者单位： | 1. 上海市刑事科学技术研究所,上海,200083
2. 复旦大学药学院药物分析教研室,上海,200032 |
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| 摘 要： | 目的建立全血中37种常见药(毒)物的固相萃取-高效液相色谱(SPE-HPLC)分析方法。方法以多沙普仑为内标,0.5mL全血经Oasis小柱固相萃取后,用HPLC进行分析,内源性物质不干扰测定。色谱柱采用LiChrospher!100RP-C18柱(250mm×4.0mm×5μm);二极管阵列检测器,检测波长为230nm和250nm,同时进行紫外扫描;柱温50℃。结果37种药(毒)物的绝对回收率除吗啡外均大于61.68%;日内及日间精密度均小于10%;检测限1～30ng/mL;线性相关系数在0.99798以上。结论本法快速、灵敏、重现性好,可用于实际案例中多种药(毒)物的分析。
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| 关 键 词： | 固相萃取 高效液相色谱法 全血 |
| 文章编号： | 1004-5619(2006)05-0349-04 |
| 修稿时间： | 2006年6月4日 |
Analysis of 37 drugs in whole blood by HPLC after solid phase extraction |
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| Liang C,Zhang YR,Jin QY,Guo YM.Analysis of 37 drugs in whole blood by HPLC after solid phase extraction[J].Journal of Forensic Medicine,2006,22(5):349-352. |
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| Authors: | Liang Chen Zhang Yu-Rong Jin Qi-Yun Guo You-Mei |
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| Institution: | Shanghai Institute of Criminal Science and Technology, Shanghai 200083, China. |
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| Abstract: | OBJECTIVE: To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood. METHODS: With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher 100 RP-C18 (250 mm x 4.0 mm x 5 microm) column kept at 50 degrees C, the DAD detector was set at 230 nm and 250 nm. RESULTS: The limit of detection were 1-30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was > or =0.997 98. The relative standard deviation for between-day and within-day assay were <10%. CONCLUSION: The method is effective, simple, reliable and has been used in real cases. |
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| Keywords: | SPE HPLC whole blood |
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