| 引起免疫失败的传染性腔上囊病病毒VP2基因在大肠杆菌中的表达 |
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| 引用本文： | 曹冰玉,王永山,范红结,欧阳伟,王忠灿,唐雨德.引起免疫失败的传染性腔上囊病病毒VP2基因在大肠杆菌中的表达[J].中国兽医科学,2010,40(2). |
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| 作者姓名： | 曹冰玉 王永山 范红结 欧阳伟 王忠灿 唐雨德 |
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| 作者单位： | 曹冰玉(江苏省农业科学院,兽医研究所,农业部动物疫病诊断与免疫重点开放实验室,国家兽用生物制品工程技术研究中心,江苏,南京,210014;南京农业大学,动物医学院,江苏,南京,210095);王永山,欧阳伟(江苏省农业科学院,兽医研究所,农业部动物疫病诊断与免疫重点开放实验室,国家兽用生物制品工程技术研究中心,江苏,南京,210014);范红结(南京农业大学,动物医学院,江苏,南京,210095);王忠灿,唐雨德(南京军区军事医学研究所,江苏,南京,210002) |
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| 基金项目： | 国家自然科学基金项目，江苏省自然科学基金项目，江苏省农业科技创新基金项目 |
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| 摘 要： | 将从安徽省传染性腔上囊病(IBD)免疫失败的病鸡腔上囊组织中扩增的VP2基因定向克隆入原核表达载体pGEX-4T-1中,构建了原核表达质粒pGEX-VP2,并转化大肠杆菌Rosetta(DE3);经IPTG诱导后,用IBDV双抗体夹心ELISA检测,结果呈阳性反应;SDS-PAGE分析结果显示,重组VP2融合蛋白的分子质量为68ku,表达量约占菌体总蛋白量的16.0%,表达产物主要以包涵体形式存在于菌体内;Western-blotting结果显示,重组VP2蛋白与IBDV抗体发生反应,形成一条特异性蛋白带,表明获得的重组蛋白具有良好的抗原反应性。
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| 关 键 词： | 传染性腔上囊病病毒 VP2基因 原核表达 |
Expression in Escherichia coli of VP2 gene of infectious bursal disease virus isolated from chickens with immunoprophylaxis defeat |
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| CAO Bing-yu,WANG Yong-shan,FAN Hong-jie,OUYANG Wei,WANG Zhong-can ,TANG Yu-de.Expression in Escherichia coli of VP2 gene of infectious bursal disease virus isolated from chickens with immunoprophylaxis defeat[J].Veterinary Science in China,2010,40(2). |
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| Authors: | CAO Bing-yu WANG Yong-shan FAN Hong-jie OUYANG Wei WANG Zhong-can TANG Yu-de |
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| Institution: | CAO Bing-yu1,2,WANG Yong-shan1,FAN Hong-jie2,OUYANG Wei1,WANG Zhong-can 3,TANG Yu-de 3(1.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Animal Disease Diagnosis , Immunology of the Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China,2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,3.Military Medical Institute of Nanjing Comm,,Nanjing 210002,China) |
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| Abstract: | A VP2 gene of infectious bursal disease virus(IBDV) was amplified from bursa of Fabricius of chickens with immunoprophylaxis defeat in Anhui Province and cloned into the prokaryotic expression vector pGEX-4T-1.The expression plasmid pGEX-VP2 was constructed and transformed into competent Escherichia coli Rosetta(DE3),then the target protein was expressed under the induction with IPTG and was positive in the detection of IBDV sandwich ELISA.SDS-PAGE analysis showed that the VP2 fusion protein was approximate... |
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| Keywords: | infectious bursal disease virus VP2 gene prokaryotic expression |
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