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| 复合扩增20个Y-STR基因座及其法医学的应用 | |
| 引用本文： | 姜先华,贾菲,沈红缨,郭飞,金萍,孙竹.复合扩增20个Y-STR基因座及其法医学的应用[J].中国法医学杂志,2013,28(1):18-22. |
| 作者姓名： | 姜先华 贾菲 沈红缨 郭飞 金萍 孙竹 |
| 作者单位： | 1. 辽宁省刑事科学技术研究所,辽宁沈阳,110032 2. 中国医科大学法医学院,辽宁沈阳,110002 3. 基点认知技术(北京)有限公司,北京,100190 |
| 摘 要： | 目的建立20个Y-STR基因座的复合扩增体系,进行遗传多态性调查,并评价其法医学应用价值。方法采用五色荧光素标记技术,对20个Y-STR基因座(DYS391、DYS389Ⅰ、DYS390、DYS389Ⅱ、DYS438、DYS460、Y GATA H4、DYS456、DYS439、DYS635、DYS448、DYS393、DYS388、DYS437、DYS19、DYS392、DYS458、DYS447、DYS385 a/b)进行复合扩增和毛细管电泳检测;调查辽宁汉族376名无关男性个体20个Y-STR基因座的遗传多态性数据;并对系统性能进行检测。结果本文方法同时检测20个Y-STR基因座,在376名个体中共检出376种单倍型,基因多样性在0.371 1～0.969 8之间;方法特异性好,分型结果准确稳定,灵敏度达0.062 5ng,实际案例常见生物检材的检验结果良好。结论20个Y-STR基因座复合扩增检测法可以用于实际案例检验,调查所获数据对建立Y-STR数据库和相关研究和应用具有重要意义。 |
| 关 键 词： | 法医物证学 Y-STR 20个基因座 复合PCR 遗传多态性 单倍型 |
A 20 Y-STR loci multiplex-PCR assay in forensic science |
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| Institution: | (Jiang Xianhua1,Jia Fei1,Shen Hongying1,Guo Fei2,Jin Ping3,Sun Zu3/1.Liaoning Criminal Science and Technology Institute,Shenyang 110032,China;2.China Medical University School of Forensic Medicine,Shenyang 110002,China;3.Peoplespot Inc,Beijing 100190,China) |
| Abstract: | Objective To construct a 20 Yplex-PCR system to aid human identity testing in forensic science and investigate the genetic polymorphisms of 20 Y-STR loci in Liaoning Han population.Methods Constructing PCR multiplex using a fivecolor fluorescence labeling 20 Y-STR loci(DYS391,DYS389Ⅰ,DYS390,DYS389 Ⅱ,DYS438,DYS460,Y GATA H4,DYS456,DYS439,DYS635,DYS448,DYS393,DYS388,DYS437,DYS19,DYS392,DYS458,DYS447 and DYS385 a/b),and validating its applicability of various biological samples and haplotype frequencies of unrelated 376 male samples in Liaoning Han population.Results The 20 Yplex-PCR system possesses the qualities of stable and accurate typing,high species-specificity and sensitivity,and well balance.It meets the needs of a variety of routine biological samples.A total of 376 different haplotypes was based on genotype diversity between 0.371 1 and 0.969 8.Conclusion The 20 Yplex-PCR system and its genetic data obtained is significant for Y-STR database establishment,population genetics studies,and forensic sciences practices. |
| Keywords: | forensic biological evidence Y-STR 20 loci PCR multiplex genetic polymorphism haplotype |
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