云南壮族人群15个STR基因座遗传多态性

目的 对600个云南壮族人群15个常染色体短串联重复(short tandem repeat,STR)序列基因座的遗传多态性进行调查,探讨云南壮族群体遗传学特性及在法医学中的应用价值.方法 使用AmpFLSTR? Identifiler? Direct PCR扩增试剂盒对此地区600名壮族无关个体血样DNA进行PCR扩...

Genetic Polymorphism of 15 Autosomal STR Loci in the Zhuang Ethnic Minority from Yunnan Province

Objective To investigate the genetic polymorphisms of 15 short tandem repeat(STR)loci in 600 Yunnan Zhuang population,and to explore the genetic characteristics of Zhuang population in Yunnan and its application value in forensic science.Methods AmpFLSTR^■Identifiler^■Direct PCR Amplification Kit was used for PCR amplification of blood samples from 600 Zhuang-independent individuals in this region.Allele frequencies and forensic parameters(observation of heterozygosity,expected heterozygosity,individual identification Rate,polymorphic information content)were calculated using Modified Powerstats software,using Arlequin 3.5 software to test the Hardy-Weinberg equilibrium and linkage disequilibrium of each locus,and then compare the genetic distance of other groups.Results The discriminative power(DP)values of various Zyx loci in Yunnan were distributed between 0.7790 and 0.9744.The cumulative individual recognition probability(CDP)reached 0.99999999999999999156.The non-parent exclusion rate ranged from 0.2869 to 0.7213.The genetic distance between Yunnan Zhuang and Guizhou Buyi,Guizhou Yi,Guangxi Maonan and Guangxi Dai is relatively close.Conclusion The 15 STR loci of this kit are highly polymorphic in Yunnan Zhuang population,which can be used for forensic identification and paternity identification.It can also be used for group genetics ethnic migration,linguistic branching,and sociological customs.whice can Provide basic research data.