| 人类DRB基因单核苷酸多态性研究 |
| |
| 引用本文： | 李军,姜先华.人类DRB基因单核苷酸多态性研究[J].中国法医学杂志,2004,19(1):9-12. |
| |
| 作者姓名： | 李军 姜先华 |
| |
| 作者单位： | 辽宁省刑事科学技术研究所,辽宁,沈阳,110032 |
| |
| 摘 要： | 目的建立一种对单核苷酸多态性(SNPs)位点进行复合检测的方法,并对人类DRB基因的单核苷酸多态性进行研究。方法 应用荧光标记双脱氧核苷酸进行单碱基延伸反应,同步检测人类DRB基因10个单核苷酸多态性位点。结果 应用该方法检验了人类标准细胞株K562、9947A及其他法医学常见生物检材,包括血斑2份、精斑5份、烟蒂8份、毛发3份,各样品间未发现相同的单核苷酸多态性组合单倍型。DNA模板需要量仅为0.5~1.0ng。结论 该方法是对微量生物物证进行个体识别鉴定的理想方法。同时还可为法医学及其他研究领域进行单核苷酸多态性分析提供快速、高效的技术手段。
|
| 关 键 词： | 法医物证学 单核苷酸多态性(SNPs) DRB基因 微量物证 |
| 文章编号： | 1001-5728(2004)01-0009-04 |
| 修稿时间： | 2003年12月1日 |
Study of Single Nucleotide Polymorphism (SNPs) of DRB |
| |
| Institution: | ( LI Jun,JIANG Xian-hua. /Liaoning Criminal Science & Technology Institute,Shenyang 110032,China) |
| |
| Abstract: | Objective Establishment of a new multiplex method for single nucleotides polymorphism study of SNPs in human DRB locus. Methods Using fluorescent dye labeled ddNTP, the single nucleotide extension (SNE) reaction was conducted to analyze 10 SNP loci in human DRB gene. Results This method was applied to examination of human standard cell line K562, 9947 A and other forensic common samples including blood stain, sperm stain, cigarette butt, hair. No identical SNPs result was shared by two samples. Conclusions Method established is ideal for DNA examination of trace biological evidence, which offers a rapid and effective tool for SNPs analysis in forensic science and other fields. |
| |
| Keywords: | Forensic biological evidnce Single nucleotide polymorphism DRB locus Trace biological evidence |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
