| 毛细管电泳高频电导法检测饮料中γ-羟基丁酸 |
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| 引用本文： | 潘爱华,盖连磊,马贤慧.毛细管电泳高频电导法检测饮料中γ-羟基丁酸[J].中国法医学杂志,2010,25(2):91-93. |
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| 作者姓名： | 潘爱华 盖连磊 马贤慧 |
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| 作者单位： | 中山大学中山医学院法医系,广东,广州,510080 |
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| 基金项目： | 广东省自然科学基金资助项目(6300649) |
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| 摘 要： | 目的建立饮料中γ-羟基丁酸（GHB）的毛细管电泳高频电导法检测分析方法。方法样品经稀释后直接进样,采用反向分离模式,以0.5mmol/LNa3PO4＋1.5mmol/LNa2HPO4＋0.1mmol/LCTAB为电泳分离缓冲介质,分离电压为16kV,进样时间为20s进行电泳分离及高频电导检测。结果该实验条件下,6.5min内可实现样品快速分析。GHB在10.0～150μg/mL范围内线性关系良好,r=0.990,检出限为3.0μg/mL（S/N=3）。选择的3种饮料样本不同添加浓度日间和日内RSD均小于6%,回收率均在95%以上。结论采用本文方法,样品处理简单,方法快速、灵敏,操作方便,可作为饮料中GHB的一种快速筛选法在相关检测中选用。
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| 关 键 词： | 法医毒物分析 毛细管电泳 高频电导检测 γ-羟基丁酸 |
Rapid determination of GHB in beverages by capillary electrophoresis with high frequency conductivity detection |
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| PAN Aihua,GAI Lianlei,MA Xianhui.Rapid determination of GHB in beverages by capillary electrophoresis with high frequency conductivity detection[J].Chinese Journal of Forensic Medicine,2010,25(2):91-93. |
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| Authors: | PAN Aihua GAI Lianlei MA Xianhui |
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| Institution: | PAN Aihua;GAI Lianlei;MA Xianhui/Department of Forensic Medicine;Zhongshan School of Medicine;Sun Yat-sen University;Guangzhou 510080;China |
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| Abstract: | Objective To establish a fast screening method based on capillary electrophoresis with high frequency conductivity detection for the determination of GHB in beverages.Methods An electrolyte consisting of 0.5mmol/L Na3PO4,1.5mmol/L Na2HPO4 and 0.1mmol/L CTAB was applied to separate the drinking samples after they had been simply diluted with water,and the seperation was carried out at voltage of 16kV using CTAB to reverse electroosmotic flow.Results With the optimized condition,the analyte could be baseline ... |
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| Keywords: | forensic toxicology analysis capillary electrophoresis high frequency conductivity detec-tion |
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