Fn-EIIIA金标试纸条进行损伤时间推断的效果评价

目的评价Fn-EIIIA金标单克隆抗体试纸条在人体皮肤组织损伤时间推断中的有效性和灵敏度。方法提取不同损伤时间人体皮肤组织,匀浆,用Fn-EIIIA金标单克隆抗体试纸条检测其Fn-EIIIA的表达,对其结果进行评价;同时,将大鼠挫伤皮肤组织置于自然环境中,分别提取不同自溶时间段的皮肤样本进行检测,对自溶变化对检测结果的影响进行观察。结果正常皮肤组织样本中试纸条检测线不显色,损伤1h后即显色,随损伤时间延长,试纸条检测线有显色逐渐加深的趋势;在观察时间段内,组织自溶对检测结果无显著影响。结论Fn-EIIIA金标单克隆抗体试纸条可作为法医学损伤时间推断敏感、稳定的检测手段。

Validation studies of the test strip of colloidal gold-labeled anti-fibronectin EIIIA splicing variant for estimating the time of injury

Objective To evaluate the validation of the test strip of colloidal gold-labeled anti- Fibronectin EIIIA(Fn-EIIIA) Splicing Variant for estimating the time of injury. Methods After skin tissues with variant injury times were collected from corpses, the test strip were used to detect the expression of Fn-EIIIA and its tested results were valued; The arisen autolysis which may influence the results of test were also observed by rats models. Results In no-injured skins, no Fn-EIIIA were detected by the strip, while in all the injured skins it was detected successfully as soon as injured after 1h and it showed more obvious results with the elongating of injured time; No changes made by autolysis were found in our studied time ranges. Conclusion The test strip which used in this study showed to be a sensitive and steady method for estimating the time of injury in forensic science.