| 突触体素在弥漫性脑损伤后的表达水平变化 |
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| 作者姓名： | Fan SL Huang P Liu YF Zhang P Wang ZY |
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| 作者单位： | 1. 西安交通大学医学院法医学系,陕西,西安,710061
2. 北华大学医学院法医教研室,吉林,吉林,132001 |
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| 基金项目： | 国家自然科学基金资助项目(30471935) |
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| 摘 要： | 目的根据突触体素在脑损伤后的变化规律,研究其与弥漫性脑损伤后损伤时间之间的关系。方法垂直撞击SD大鼠造成脑损伤模型,在不同时间点处死大鼠,取脑组织冠状断面进行突触体素染色,将染色结果进行灰度值测定,并用SPSS软件对灰度值进行分析,从而推断损伤后随时间变化的突触体素变化情况。结果突触体素在损伤后一段时间内,随时间的变化呈现双拐曲线,显示突触体素表达与脑损伤及其修复过程密切相关。结论根据脑组织内突触体素的含量变化在一定范围内可以用于推断脑损伤时间。
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| 关 键 词： | 突触体素 弥漫性脑损伤 损伤时间 |
| 文章编号： | 1004-5619(2007)01-0008-03 |
| 修稿时间： | 2006年9月7日 |
Expression of synaptophysin in experimental diffuse brain injury |
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| Fan SL,Huang P,Liu YF,Zhang P,Wang ZY.Expression of synaptophysin in experimental diffuse brain injury[J].Journal of Forensic Medicine,2007,23(1):8-10,13,F0003. |
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| Authors: | Fan Shuan-liang Huang Ping Liu Yan-feng Zhang Pin Wang Zhen-yuan |
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| Institution: | Department of Forensic Science, Medical College, Xi'an Jiaotong University, Xi'an 710061, China. xafansl@mail.xjtu.edu.cn |
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| Abstract: | Object To investigate the changes in the expression_level of synaptophysin following diffuse brain injury(DBI) in rats and to correlate the changes of the synaptophysin expression_level with the post injury time interval.Methods Wister rats were used as a DBI model induced by Marmarou method.The changes of synaptophysin immunoreactivity on coronal sections of the rats sampled at different post-injury time intervals were used as a marker.The densitometry of the synaptophysin immunoreactivity was documented by imaging technique and analyzed by SPSS software.Results The expression_level of synaptophysin in DBI rats showed dynamic changes following DBI as well as during the repairing period.Conclusion The changes of synaptophysin level may be used as a marker for estimation of the post injury time interval in DBI. |
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| Keywords: | synaptophysin diffuse brain injury post injury time interval |
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