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复合扩增STR位点片段长度多态性的研究
引用本文:刘健,尤朝阳,郑秀芬,李伯龄,倪锦堂,李晓斌.复合扩增STR位点片段长度多态性的研究[J].中国法医学杂志,1994(4).
作者姓名:刘健  尤朝阳  郑秀芬  李伯龄  倪锦堂  李晓斌
作者单位:公安部第二研究所!北京,100038,公安部第二研究所!北京,100038,公安部第二研究所!北京,100038,公安部第二研究所!北京,100038,公安部第二研究所!北京,100038,公安部第二研究所!北京,100038
摘    要:本文介绍了以三个短串联重复序列(STR)位点为基础的复合扩增进行个体识别的技术。其特点是在同一扩增体系内对彼此独立的三个STR位点,即HUMTH01AATG]nHUMFABPAAT]nHUMARAAGC]n进行复会扩增,扩增产物用聚丙烯酰胺凝胶电泳分离。经研究发现这三个STR位点具有高度多态性,扩增产物分子量在190~310bP之间,各位点等位基因数分别为6、9、16个,杂合度78.2%、85,0%、89.0%,个体识别率0.918、0.960、0.905,其累积的个体识别率达0.9997,高于pMCT118等位点,且扩增时间短,操作简便,为法医物证检验提供了一种高效的个体识别手段。

关 键 词:短串联重复序列(STR)  复合扩增  扩增片段长度多态性  个体识别

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF THREE STRs LOCI IN CHINESE USING MULIPLEX PCR
Liu Jian,You Chaoyang,Zheng Xiufen,Li Boling,Ni Jintang,Li XiaobinInstitute of Forensic Science,Ministry of Public Security,Beijing.AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF THREE STRs LOCI IN CHINESE USING MULIPLEX PCR[J].Chinese Journal of Forensic Medicine,1994(4).
Authors:Liu Jian  You Chaoyang  Zheng Xiufen  Li Boling  Ni Jintang  Li XiaobinInstitute of Forensic Science  Ministry of Public Security  Beijing
Institution:Liu Jian,You Chaoyang,Zheng Xiufen,Li Boling,Ni Jintang,Li XiaobinInstitute of Forensic Science,Ministry of Public Security,Beijing 100038
Abstract:The multiplex PCR of three STRs loci {HUMTHO1 AATG] n, HUMFABP AAT] n, HUMARAAGC]n} for personal identification has been descrrbed. The PCR products were analyzed by5M Urea, 8 %PAGE,followd by silver stain. 6, 9, 16 alleles,ranging from 190bp to 310 bp in size, 78.2 %, 85. 0 %, 89. 0 % heterozygosity of three loci were demonstrated. The combined averagee individualizati0n potential was 3/10000 individuals
Keywords:Short tandem repeats(STRs)  Multiplex PCR  Amplified fragment length polymorphism(Amp-Flp)  Individual identification
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