| X染色体STR单倍型DXS7424-DXS101遗传多态性 |
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| 作者姓名： | Yu B Tuo Y Gui HS Li SB |
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| 作者单位： | 西安交通大学卫生部法医学重点实验室,环境与疾病相关基因教育部重点实验室,陕西,西安,710061 |
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| 基金项目： | 教育部科技基础平台项目:中华民族遗传资源数据整合共享平台(505015) |
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| 摘 要： | 目的研究X染色体STR单倍型DXS7424-DXS101在汉族群体中的法医学特征及其分布特点。方法本实验应用PCR和聚丙烯酰胺凝胶电泳及银染技术对DXS7424和DXS101位点进行基因分型,利用相关统计方法进行数据分析。结果汉族151个男性样本中共检出37种单倍型,频率分布在0.0066～0.1391,GD值为0.9453,DP值达到0.9389,最常见单倍型为16-23。结论DXS7424-DXS101作为单倍型分析在汉族群体中具有较高的个体识别率和非父排除率,在法医学特殊亲权鉴定中具有良好的应用前景。
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| 关 键 词： | X染色体 短串联重复序列(STR) 单倍型 多态性 |
Validation the haplotype polymorphisms of the DXS7424-DXS101 on X-chromosome |
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| Yu B,Tuo Y,Gui HS,Li SB.Validation the haplotype polymorphisms of the DXS7424-DXS101 on X-chromosome[J].Journal of Forensic Medicine,2007,23(5):342-344. |
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| Authors: | Yu Bing Tuo Ya Gui Hong-sheng Li Sheng-bin |
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| Institution: | Key Laboratory of Forensic Sciences, National Ministry of Health, Xi'an Jiaotong University, Xi'an, 710061 China. yubing@mail.xjtu.edu.cn |
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| Abstract: | Objective To validate the genetic characteristics and distribution of DXS7424-DXS101 on X chromosome in Han population. Methods DXS7424 and DXS101 loci were genotyped by PCR,PAGE and silvers stain methods. Their genetic parameters were analyzed by Arlequin software. Results There were 37 haplotypes detected in 151 Han unrelated males. The frequencies ranged from 0.006 6 to 0.139 1,with a GD value of 0.945 3 and a DP value of 0.938 9. Haplotypes 16-23 were the most common haplotypes in Han population. Conclusion Analysis of combined DXS7424 and DXS101 haplotypes appears to be a powerful means in population genetics and forensic practice for determination of identity and paternity. |
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| Keywords: | X chromosome short tandem repeats (STR) haplotype polymorphism |
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