| 根据mRNA稳定性推断死亡时间的研究 |
| |
| 作者姓名： | Xiao JH Chen YC Wang JF |
| |
| 作者单位： | 中山大学基础医学院法医病理教研室,广东,广州,510080 |
| |
| 基金项目： | 广东省自然科学基金资助项目(036628) |
| |
| 摘 要： | 目的检测大鼠肺、胸肌β肌动蛋白(β-actin)mRNA在死后不同时间的表达,为死亡时间的推断寻找新的指标。方法大鼠断颈处死后于21℃温度控制系统模拟死后改变,在不同时间点抽提相应组织总RNA,利用RT-PCR技术检测β-actinmRNA的表达水平变化并对扩增产物进行半定量分析。结果大鼠肺β-actinmRNA于死后12d仍可检出,胸肌在死后8d的检测呈阴性。β-actinmRNA在肺脏和胸肌的稳定性呈现器官差异性和时间差异性。结论观察死后肺、胸肌β-actinmRNA的变化,可为死亡时间推断提供客观依据。
|
| 关 键 词： | 死亡时间 RT-PCR β肌动蛋白 大鼠 肺脏 胸肌 |
| 文章编号： | 1004-5619(2005)01-0019-02 |
| 修稿时间： | 2004年5月9日 |
A preliminary study on estimation of postmortem interval according to beta-actin mRNA stability in rat |
| |
| Xiao JH,Chen YC,Wang JF.A preliminary study on estimation of postmortem interval according to beta-actin mRNA stability in rat[J].Journal of Forensic Medicine,2005,21(1):19-20. |
| |
| Authors: | Xiao Jun-hui Chen Yu-chuan Wang Jiang-feng |
| |
| Institution: | Department of forensic pathology, preliminary medical college of Sun Yat-Sen University, Guangzhou 510080, China. xiaoxiaopost@163.com |
| |
| Abstract: | OBJECTIVE: In order to find a new parameter to estimate the postmortem interval, beta-actin mRNA in lung and thoracic muscle of rats was detected at different time point postmortem. METHODS: Rats were killed by neck dislocation and left in a temperature controlling system at 21 degrees C for 12 days postmortem. Total RNA in lung and thoracic muscle at different time point was extracted and beta-actin mRNA expression was examined by RT-PCR. Semi-quantification analysis of the image of electrophoresis was performed to confirm the changes of beta-actin mRNA expression. RESULTS: beta-actin mRNA in lung of rats still could be detected at 12 days postmortem, but-disappeared in thoracic muscle at 8 days postmortem. CONCLUSION: The expression of beta-actin mRNA in lung and thoracic muscle could be a new parameter for estimation of PMI. |
| |
| Keywords: | RT-PCR |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
