| HLA-DRB1位点的PCR-SSP分型在亲子鉴定中的应用研究 |
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| 作者姓名： | Liu LM Liang J Wang BJ Ding M Li JP Li CM Xiao YZ Jia JT |
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| 作者单位： | 中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学第一临床学院器官移植科!辽宁沈阳,110001,中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学法医系血清学教研室!辽宁沈阳,110001,中国医科大学法医 |
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| 基金项目： | 国家教委留学归国人员科研基金!95135,辽宁省科委自然科学基金!962312 |
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| 摘 要： | 应用PCR－SSP（PCRamplificationwithsequencespecificprimer）方法将HLAⅡ类DRB1位点基因分型应用于亲权鉴定。对42例亲子鉴定案例进行分析研究的结果表明,本方法简单、快速、结果可靠,且具有较高的非父排除概率（66．3％）,不仅适用于法医学亲手鉴定和个人识别,亦可应用于移植配型、HLA相关疾病及人类遗传学研究。
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| 关 键 词： | 亲子鉴定 PCR-SSP HLA－DRB1基因 |
HLA-DRB1 typing by PCR-sequence specific primers for paternity determination |
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| Liu LM,Liang J,Wang BJ,Ding M,Li JP,Li CM,Xiao YZ,Jia JT.HLA-DRB1 typing by PCR-sequence specific primers for paternity determination[J].Journal of Forensic Medicine,1999,15(4):201-203. |
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| Authors: | Liu L M Liang J Wang B J Ding M Li J P Li C M Xiao Y Z Jia J T |
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| Institution: | Department of Forensic Medicine, China Medical University, Shenyang 110001, P. R. China. |
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| Abstract: | HLA-DRB1 typing by PCR - SSP technique was first used for patemity testing. The analysis of 42 paternity cases shows that this identification method is simple, Thst and reliable and has high exclusion probability of paternity (66. 3% ). This method can also be applied to the study of transplantation, HLA associated disease and anthropogenesis |
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| Keywords: | paternity testing PCR - SSP HLA - DRB1 gene |
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