| 温州汉族人群D7S2846、D19S400和D18S535基因座的遗传多态性研究 |
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| 作者姓名： | Zhang HQ Wu SZ Bi YT |
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| 作者单位： | 温州医学院分子生物学研究中心,浙江,温州,325027 |
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| 摘 要： | 目的研究D7S2846、D19S400和D18S535位点在温州汉族人群的遗传多态性。方法EDTA抗凝血样采自194名无血缘关系温州地区汉族个体,用chelex-100法提取DNA,PCR扩增,聚丙烯酰胺凝胶电泳,银染显色分析。结果D7S2846观察到6个等位基因及15种基因型;D19S400观察到10个等位基因及36种基因型;D18S535观察到8个等位基因及26种基因型。各基因座的杂合度(H)分别为:0.644、0.724、0.772;个人识别能力(Dp):0.854、0.940、0.938。结论三个STR基因座具有较高杂合度,等位基因分布符合Hardy-Weinberg平衡,在法医学应用和群体遗传学研究中有较高的价值。
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| 关 键 词： | 短串联重复序列 聚合酶链反应 遗传多态性 |
| 文章编号： | 1004-5619(2004)03-0160-02 |
| 修稿时间： | 2003年8月21日 |
A study of genetic polymorphism of the STR locus D7S2846, D19S400 and D18S535 in Han population in Wenzhou |
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| Zhang HQ,Wu SZ,Bi YT.A study of genetic polymorphism of the STR locus D7S2846, D19S400 and D18S535 in Han population in Wenzhou[J].Journal of Forensic Medicine,2004,20(3):160-161. |
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| Authors: | Zhang Hong-qin Wu Shu-zhen Bi Yun-tian |
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| Institution: | Molecular Biology Research Centre of Wenzhou Medical College, Wenzhou 325027, China. |
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| Abstract: | OBJECTIVE: To study genetic polymorphism of D7S2846, D19S400 and D18S535 in Han population in Wenzhou. METHODS: DNA was extracted with chelex-100 method from EDTA-blood samples of 194 unrelated individuals in Wenzhou and amplified with PCR technique. The PCR products were analyzed by PAG vertical electrophoresis and silver-stain. RESULTS: 6 alleles and 15 genotypes of D7S2846, 10 alleles and 36 genotypes of D19S400, 8 alleles and 26 genotypes of D18S535 was observed. The heterozygosities of the three loci are 0.644, 0.724 and 0.772; The power of discriminating are 0.854, 0.940 and 0.938. CONCLUSION: The heterozygosities of the three loci are high and the frequencies was in accordance with Hardy-Weinberg equilibrium (P>0.05), so the three loci can be used in forensic medicine and in other genetic researches. |
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| Keywords: | short tandem repeats polymerase chain reaction genetic polymorphism |
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