| 青岛地区汉族人群13个STR基因座的频率分布及法医学应用 |
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| 引用本文： | 李志强,李永红,李震海,赵彩霞,纪尚起,接贵祥,于丰钦.青岛地区汉族人群13个STR基因座的频率分布及法医学应用[J].法律与医学杂志,2002,9(4):219-222. |
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| 作者姓名： | 李志强 李永红 李震海 赵彩霞 纪尚起 接贵祥 于丰钦 |
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| 作者单位： | 青岛市中级人民法院 266071
(李志强，赵彩霞，纪尚起，接贵祥)，青岛大学医学院附属医院急诊内科 266003
(李永红)，青岛市卫生局卫生监督所 266033
(李震海)，青岛市中级人民法院 266071(于丰钦) |
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| 摘 要： | 目的 调查青岛地区汉族人群无关个体的 13个STR基因座 (D3S135 8、VWA、FGA、D8S1179、D2 1S11、D18S5 1、D5S818、D13S317、D7S82 0、D16S5 39、TH0 1、TPOX、CSFIPO)的基因频率分布 ,研究其遗传多态性及其在法医学个体识别及亲子鉴定中的应用价值。 方法 用美国ABI - 310型遗传分析仪对ProfilerPlus和Cofiler两个系统的 13个STR基因座的复合扩增产物进行毛细管电泳及四色荧光自动分析检测 ,基因分型软件为GeneScanv3.1和Genotyperv2 .5 .2。 结果 获得 13个STR基因座在青岛地区汉族人群的基因频率分布数据 ,13个STR基因座的PIC >0 .5 ,DP >0 .71,CCE =0 .999999,TDP值接近 1,TPm =1.2× 10 -14 ,家系调查符合孟德尔遗传规律。 结论 ProfilerPlus和Cofiler两个系统的 13个STR基因座在法医学个体识别及亲子鉴定中具有较高的应用价值。
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| 关 键 词： | 荧光标记 复合扩增 STR基因座 遗传多态性 亲子鉴定 |
| 文章编号： | 1007-9297(2002)04-0219-04 |
| 修稿时间： | 2002年5月23日 |
Experimental study on genetic polymorphisms and forensic applications of 13 STR loci in Qingdao Han population. |
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| LI Zhi-qiang,LI Yong-hong,LI Zhen-hai,et al..Experimental study on genetic polymorphisms and forensic applications of 13 STR loci in Qingdao Han population.[J].The Journal of Law & Medicine,2002,9(4):219-222. |
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| Authors: | LI Zhi-qiang LI Yong-hong LI Zhen-hai |
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| Institution: | LI Zhi-qiang,LI Yong-hong,LI Zhen-hai,et al. The People's middle Court of Qingdao. Qingdao. 266071 |
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| Abstract: | Objective To investigate the genetic polymorphisms and forensic applications of 13 STR loci(D3S1358?VWA?FGA?D8S1179?D21S11?D18S51?D5S818?D13S317?D7S820?D16S539?TH01?TPOX?CSFIPO), and obtain the alleles frequencies and genetic data of 13 STR loci in Chinese Han population of Qingdao area. Methods Utilizing the Profiler Plus and Cofiler kit, the 13 STR loci were amplified on DNA samples extracted from EDTA-blood of 419 unrelated individuals. The fluorescently tagged multiplex polymerase chain reaction (PCR) were carried out. The PCR products were analyzed andgenotyped by ABI-310 Sequencer. Results The population genetic data were obtained and the alleles frequencies of 13 STR loci in Chinese Han population of Qingdao area were determined. No deviations from Hardy-Weinberg equilibrium hypothesis were detected for these 13 STR loci. Conclusion The 13 STR loci were very useful in forensic practice. |
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| Keywords: | Fluorescent tag Multiplexing PCR STR loci Genetic polymorphism Forensic science |
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