| 传染性羊痒病抗性基因分型方法的建立 |
| |
| 引用本文： | 董志珍,赵祥平,侯艳梅,李琳,肖妍,蔡国瑞,栾慎顺,霍蕾.传染性羊痒病抗性基因分型方法的建立[J].中国兽医科学,2010,40(3). |
| |
| 作者姓名： | 董志珍 赵祥平 侯艳梅 李琳 肖妍 蔡国瑞 栾慎顺 霍蕾 |
| |
| 作者单位： | 天津出入境检验检疫局,天津,300456 |
| |
| 基金项目： | 国家质检总局科技计划项目 |
| |
| 摘 要： | 根据GenBank中登录的绵羊朊蛋白编码基因PRNP序列,设计并合成了可用于单核苷酸多态性(SNP)快速分析的3对引物和7条分别标记了FAM和VIC荧光染料的MGB探针,建立了一种利用荧光定量PCR扩增反应对羊痒病抗性基因进行筛选的方法。结果表明,设计的引物及探针具有特异性和高效扩增性,能够用于PRNP羊痒病抗性基因的快速分型。借助该方法可建立一整套完善的预警监测体系来预防该病的发生,也可用于羊痒病的常规实验室诊断。
|
| 关 键 词： | 传染性羊瘁病 朊蛋白基因 羊痒病抗性基因 基因分型 |
Establishment of a method for genotyping scrapie-resistant genes |
| |
| DONG Zhi-zhen,ZHAO Xiang-ping,HOU Yan-mei,LI Lin,XIAO Yan,CAI Guo-rui,LUAN Shen-shun,HUO Lei.Establishment of a method for genotyping scrapie-resistant genes[J].Veterinary Science in China,2010,40(3). |
| |
| Authors: | DONG Zhi-zhen ZHAO Xiang-ping HOU Yan-mei LI Lin XIAO Yan CAI Guo-rui LUAN Shen-shun HUO Lei |
| |
| Institution: | DONG Zhi-zhen,ZHAO Xiang-ping,HOU Yan-mei,LI Lin,XIAO Yan,CAI Guo-rui,LUAN Shen-shun,HUO Lei(Tianjin Import-Export Inspection , Quarantine Bureau,Tianjin 300456,China) |
| |
| Abstract: | Three pairs of primers and 7 strips of MGB TaqMan probes marked with FAM and VIC were respectively designed according to the sequences of prion protein genes in GenBank,and used for analysis of single nucleotide polymorphism after real-time PCR amplification.In result,the developed method was specific and high-efficient,and could be used genotype scrapie-resistant genes.The results showed that the developed method,an early-warning and monitoring system would be established to prevent the outbreak of scrapie... |
| |
| Keywords: | transmissible scrapie prion protein gene scrapie-resistant gene genotyping |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
